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Literature summary for 2.3.1.38 extracted from

  • Shimakata, T.; Stumpf, P.K.
    The purification and function of acetyl coenzyme A:acyl carrier protein transacylase (1983), J. Biol. Chem., 258, 3592-3598.
    View publication on PubMed
Search for more literature for 2.3.1.38

Inhibitors

Inhibitors Comment Organism Structure
arsenite
-
 Spinacia oleracea
N-ethylmaleimide
-
 Spinacia oleracea
p-Substituted mercuribenzoate
-
 Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 acetyl-CoA
-
 Spinacia oleracea
0.0083
-
 butyryl-CoA
-
 Spinacia oleracea
0.0086
-
 hexanoyl-CoA
-
 Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + [acyl-carrier protein] Carthamus tinctorius
-
 CoA + acetyl-[acyl-carrier protein]
-
 ?
acetyl-CoA + [acyl-carrier protein] Spinacia oleracea increase in enzyme level in reconsituted FAS system enhances production of medium length fatty acids CoA + acetyl-[acyl-carrier protein]
-
 ?
acetyl-CoA + [acyl-carrier protein] Spinacia oleracea rate-limiting step of fatty acid synthesis CoA + acetyl-[acyl-carrier protein]
-
 ?

Organism

Organism UniProt Comment Textmining
Brassica juncea
-
-
-
Carthamus tinctorius
-
 safflower
-
Cuphea lutea
-
-
-
Lathyrus oleraceus
-
-
-
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-
leaf
-
 Lathyrus oleraceus
-
seed
-
 Cuphea lutea
-
seed
-
 Carthamus tinctorius
-
seed
-
 Brassica juncea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Cuphea lutea
additional information
-
-
 Carthamus tinctorius
additional information
-
-
 Brassica juncea
additional information
-
-
 Lathyrus oleraceus
additional information
-
 fatty acid synthase II assay Spinacia oleracea
0.000005
-
-
 Lathyrus oleraceus
0.000009
-
 crude enzyme extract Spinacia oleracea
0.000018
-
-
 Carthamus tinctorius
0.000064
-
-
 Cuphea lutea
0.00019
-
-
 Brassica juncea
0.0056
-
 purified enzyme Spinacia oleracea

Storage Stability

Storage Stability Organism
-70°C, several months Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + [acyl-carrier protein]
-
 Spinacia oleracea CoA + acetyl-[acyl-carrier protein]
-
 ?
acetyl-CoA + [acyl-carrier protein]
-
 Lathyrus oleraceus CoA + acetyl-[acyl-carrier protein]
-
 ?
acetyl-CoA + [acyl-carrier protein]
-
 Brassica juncea CoA + acetyl-[acyl-carrier protein]
-
 ?
acetyl-CoA + [acyl-carrier protein]
-
 Carthamus tinctorius CoA + acetyl-[acyl-carrier protein]
-
 ?
acetyl-CoA + [acyl-carrier protein]
-
 Cuphea lutea CoA + acetyl-[acyl-carrier protein]
-
 ?
acetyl-CoA + [acyl-carrier protein] increase in enzyme level in reconsituted FAS system enhances production of medium length fatty acids Spinacia oleracea CoA + acetyl-[acyl-carrier protein]
-
 ?
acetyl-CoA + [acyl-carrier protein] rate-limiting step of fatty acid synthesis Spinacia oleracea CoA + acetyl-[acyl-carrier protein]
-
 ?
butyryl-CoA + [acyl-carrier protein]
-
 Spinacia oleracea CoA + butyryl-[acyl-carrier protein]
-
 ?
hexanoyl-CoA + [acyl-carrier protein]
-
 Spinacia oleracea CoA + hexanoyl-[acyl-carrier protein]
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
 assay at Brassica juncea
38
-
 assay at Carthamus tinctorius
38
-
 assay at Cuphea lutea
38
-
 assay at Lathyrus oleraceus
38
-
 assay at Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
-
 Spinacia oleracea