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Literature summary for 2.3.1.37 extracted from

  • Stoian, N.; Kaganjo, J.; Zeilstra-Ryalls, J.
    Resolving the roles of the Rhodobacter sphaeroides HemA and HemT 5-aminolevulinic acid synthases (2018), Mol. Microbiol., 110, 1011-1029 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BW25113 cells Cereibacter sphaeroides

Protein Variants

Protein Variants Comment Organism
C281P the mutant shows about wild type activity. In the presence of 0.001 mM hemin, mutant C281P of isoform HemT retains 100% of its activity Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
hemin in the presence of 0.001 mM hemin, wild type isoform HemA retains 69% of its activity; in the presence of 0.001 mM hemin, wild type isoform HemT retains 90% of its activity while isoform HemA retains 62% of its activity Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0146
-
succinyl-CoA isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
0.015
-
succinyl-CoA isoform HemT, pH and temperature not specified in the publication Cereibacter sphaeroides
0.0176
-
succinyl-CoA isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
0.0184
-
succinyl-CoA isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
0.0261
-
succinyl-CoA mutant C281P of isoform HemT, pH and temperature not specified in the publication Cereibacter sphaeroides
26.4
-
glycine isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
31.7
-
glycine isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
43.4
-
glycine isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
46.8
-
glycine mutant C281P of isoform HemT, pH and temperature not specified in the publication Cereibacter sphaeroides
52.5
-
glycine isoform HemT, pH and temperature not specified in the publication Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinyl-CoA + glycine Cereibacter sphaeroides
-
5-aminolevulinate + CoA + CO2
-
?
succinyl-CoA + glycine Cereibacter sphaeroides ATCC17023
-
5-aminolevulinate + CoA + CO2
-
?
succinyl-CoA + glycine Cereibacter sphaeroides ATCC17029
-
5-aminolevulinate + CoA + CO2
-
?
succinyl-CoA + glycine Cereibacter sphaeroides ATCC17025
-
5-aminolevulinate + CoA + CO2
-
?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
isolate 2.4.3
-
Cereibacter sphaeroides
-
isolate 2.4.9
-
Cereibacter sphaeroides Q04512 isolate 2.4.1
-
Cereibacter sphaeroides ATCC17023 Q04512 isolate 2.4.1
-
Cereibacter sphaeroides ATCC17025
-
isolate 2.4.3
-
Cereibacter sphaeroides ATCC17029
-
isolate 2.4.9
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.05
-
isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
1.05
-
isoform HemT, pH and temperature not specified in the publication Cereibacter sphaeroides
1.07
-
mutant C281P of isoform HemT, pH and temperature not specified in the publication Cereibacter sphaeroides
1.13
-
isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
1.89
-
isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + glycine
-
Cereibacter sphaeroides 5-aminolevulinate + CoA + CO2
-
?
succinyl-CoA + glycine
-
Cereibacter sphaeroides ATCC17023 5-aminolevulinate + CoA + CO2
-
?
succinyl-CoA + glycine
-
Cereibacter sphaeroides ATCC17029 5-aminolevulinate + CoA + CO2
-
?
succinyl-CoA + glycine
-
Cereibacter sphaeroides ATCC17025 5-aminolevulinate + CoA + CO2
-
?

Synonyms

Synonyms Comment Organism
5-aminolevulinic acid synthase
-
Cereibacter sphaeroides
ALA synthase
-
Cereibacter sphaeroides
hemA isoform Cereibacter sphaeroides
HemT isoform Cereibacter sphaeroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.01
-
glycine isoform HemT, pH and temperature not specified in the publication Cereibacter sphaeroides
1.01
-
succinyl-CoA isoform HemT, pH and temperature not specified in the publication Cereibacter sphaeroides
1.06
-
glycine isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
1.06
-
succinyl-CoA isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
1.1
-
glycine isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
1.1
-
succinyl-CoA isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
1.3
-
glycine isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
1.3
-
succinyl-CoA isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
55.48
-
glycine mutant C281P of isoform HemT, pH and temperature not specified in the publication Cereibacter sphaeroides
55.48
-
succinyl-CoA mutant C281P of isoform HemT, pH and temperature not specified in the publication Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Cereibacter sphaeroides

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0006
-
hemin estimated value, isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
0.008
-
hemin estimated value, isoform HemT, pH and temperature not specified in the publication Cereibacter sphaeroides

Expression

Organism Comment Expression
Cereibacter sphaeroides hemT expression is strongly upregulated under anaerobic-dark growth conditions when cells are respiring dimethyl sulfoxide, versus aerobic-dark or phototrophic (anaerobic-light) conditions up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.019
-
glycine isoform HemT, pH and temperature not specified in the publication Cereibacter sphaeroides
0.02
-
glycine mutant C281P of isoform HemT, pH and temperature not specified in the publication Cereibacter sphaeroides
0.026
-
glycine isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
0.033
-
glycine isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
0.049
-
glycine isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
35.4
-
succinyl-CoA mutant C281P of isoform HemT, pH and temperature not specified in the publication Cereibacter sphaeroides
62.5
-
succinyl-CoA isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
67.2
-
succinyl-CoA isoform HemT, pH and temperature not specified in the publication Cereibacter sphaeroides
70.3
-
succinyl-CoA isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides
72.4
-
succinyl-CoA isoform HemA, pH and temperature not specified in the publication Cereibacter sphaeroides