Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BW25113 cells | Cereibacter sphaeroides |
Protein Variants | Comment | Organism |
---|---|---|
C281P | the mutant shows about wild type activity. In the presence of 0.001 mM hemin, mutant C281P of isoform HemT retains 100% of its activity | Cereibacter sphaeroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hemin | in the presence of 0.001 mM hemin, wild type isoform HemA retains 69% of its activity; in the presence of 0.001 mM hemin, wild type isoform HemT retains 90% of its activity while isoform HemA retains 62% of its activity | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0146 | - |
succinyl-CoA | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
0.015 | - |
succinyl-CoA | isoform HemT, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
0.0176 | - |
succinyl-CoA | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
0.0184 | - |
succinyl-CoA | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
0.0261 | - |
succinyl-CoA | mutant C281P of isoform HemT, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
26.4 | - |
glycine | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
31.7 | - |
glycine | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
43.4 | - |
glycine | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
46.8 | - |
glycine | mutant C281P of isoform HemT, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
52.5 | - |
glycine | isoform HemT, pH and temperature not specified in the publication | Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | Cereibacter sphaeroides | - |
5-aminolevulinate + CoA + CO2 | - |
? | |
succinyl-CoA + glycine | Cereibacter sphaeroides ATCC17023 | - |
5-aminolevulinate + CoA + CO2 | - |
? | |
succinyl-CoA + glycine | Cereibacter sphaeroides ATCC17029 | - |
5-aminolevulinate + CoA + CO2 | - |
? | |
succinyl-CoA + glycine | Cereibacter sphaeroides ATCC17025 | - |
5-aminolevulinate + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
isolate 2.4.3 | - |
Cereibacter sphaeroides | - |
isolate 2.4.9 | - |
Cereibacter sphaeroides | Q04512 | isolate 2.4.1 | - |
Cereibacter sphaeroides ATCC17023 | Q04512 | isolate 2.4.1 | - |
Cereibacter sphaeroides ATCC17025 | - |
isolate 2.4.3 | - |
Cereibacter sphaeroides ATCC17029 | - |
isolate 2.4.9 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.05 | - |
isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides |
1.05 | - |
isoform HemT, pH and temperature not specified in the publication | Cereibacter sphaeroides |
1.07 | - |
mutant C281P of isoform HemT, pH and temperature not specified in the publication | Cereibacter sphaeroides |
1.13 | - |
isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides |
1.89 | - |
isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | - |
Cereibacter sphaeroides | 5-aminolevulinate + CoA + CO2 | - |
? | |
succinyl-CoA + glycine | - |
Cereibacter sphaeroides ATCC17023 | 5-aminolevulinate + CoA + CO2 | - |
? | |
succinyl-CoA + glycine | - |
Cereibacter sphaeroides ATCC17029 | 5-aminolevulinate + CoA + CO2 | - |
? | |
succinyl-CoA + glycine | - |
Cereibacter sphaeroides ATCC17025 | 5-aminolevulinate + CoA + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-aminolevulinic acid synthase | - |
Cereibacter sphaeroides |
ALA synthase | - |
Cereibacter sphaeroides |
hemA | isoform | Cereibacter sphaeroides |
HemT | isoform | Cereibacter sphaeroides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.01 | - |
glycine | isoform HemT, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
1.01 | - |
succinyl-CoA | isoform HemT, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
1.06 | - |
glycine | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
1.06 | - |
succinyl-CoA | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
1.1 | - |
glycine | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
1.1 | - |
succinyl-CoA | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
1.3 | - |
glycine | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
1.3 | - |
succinyl-CoA | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
55.48 | - |
glycine | mutant C281P of isoform HemT, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
55.48 | - |
succinyl-CoA | mutant C281P of isoform HemT, pH and temperature not specified in the publication | Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Cereibacter sphaeroides |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
hemin | estimated value, isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
0.008 | - |
hemin | estimated value, isoform HemT, pH and temperature not specified in the publication | Cereibacter sphaeroides |
Organism | Comment | Expression |
---|---|---|
Cereibacter sphaeroides | hemT expression is strongly upregulated under anaerobic-dark growth conditions when cells are respiring dimethyl sulfoxide, versus aerobic-dark or phototrophic (anaerobic-light) conditions | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
glycine | isoform HemT, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
0.02 | - |
glycine | mutant C281P of isoform HemT, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
0.026 | - |
glycine | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
0.033 | - |
glycine | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
0.049 | - |
glycine | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
35.4 | - |
succinyl-CoA | mutant C281P of isoform HemT, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
62.5 | - |
succinyl-CoA | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
67.2 | - |
succinyl-CoA | isoform HemT, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
70.3 | - |
succinyl-CoA | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
72.4 | - |
succinyl-CoA | isoform HemA, pH and temperature not specified in the publication | Cereibacter sphaeroides |