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Literature summary for 2.3.1.37 extracted from

  • Tan, Z.; Zhao, J.; Chen, J.; Rao, D.; Zhou, W.; Chen, N.; Zheng, P.; Sun, J.; Ma, Y.
    Enhancing thermostability and removing hemin inhibition of Rhodopseudomonas palustris 5-aminolevulinic acid synthase by computer-aided rational design (2019), Biotechnol. Lett., 41, 181-191 .
    View publication on PubMed
Search for more literature for 2.3.1.37

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells and Corynebacterium glutamicum strain ATCC 13032 Rhodopseudomonas palustris

Protein Variants

Protein Variants Comment Organism
H15K the mutant exhibits a 6.0°C higher melting temperature and shows a 10.3fold increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
H15R the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
H176D the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
H206F the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
H206Y the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
H216D inactive Rhodopseudomonas palustris
H236D the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
H293Y the mutant shows a decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
H29R the mutant exhibits a 2.3°C higher melting temperature and shows a 6.7fold increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
H304M the mutant shows a strong increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
H304Q the mutant shows a decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
H304V the mutant shows a decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
H322L the mutant shows a severe decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
H322V the mutant shows a severe decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
H385R the mutant shows a decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
H39Y the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
H61D the mutant shows a severe decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
H61N the mutant shows a decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris

Inhibitors

Inhibitors Comment Organism Structure
hemin 27% residual activity at 0.01 mM Rhodopseudomonas palustris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01513
-
 succinyl-CoA mutant enzyme H15K, at pH 7.5 and 37°C Rhodopseudomonas palustris
0.0214
-
 succinyl-CoA wild type enzyme, at pH 7.5 and 37°C Rhodopseudomonas palustris
0.02227
-
 succinyl-CoA mutant enzyme H29R, at pH 7.5 and 37°C Rhodopseudomonas palustris
2.31
-
 glycine wild type enzyme, at pH 7.5 and 37°C Rhodopseudomonas palustris
2.63
-
 glycine mutant enzyme H29R, at pH 7.5 and 37°C Rhodopseudomonas palustris
3.25
-
 glycine mutant enzyme H15K, at pH 7.5 and 37°C Rhodopseudomonas palustris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinyl-CoA + glycine Rhodopseudomonas palustris
-
 5-aminolevulinate + CoA + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodopseudomonas palustris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography Rhodopseudomonas palustris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + glycine
-
 Rhodopseudomonas palustris 5-aminolevulinate + CoA + CO2
-
 ?

Synonyms

Synonyms Comment Organism
ALA synthase
-
 Rhodopseudomonas palustris
ALAS
-
 Rhodopseudomonas palustris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 after 60 min incubation at 37°C, the residual activity of wild type dramatically drops to 8% of the original activity Rhodopseudomonas palustris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.74
-
 succinyl-CoA wild type enzyme, at pH 7.5 and 37°C Rhodopseudomonas palustris
3.1
-
 succinyl-CoA mutant enzyme H15K, at pH 7.5 and 37°C Rhodopseudomonas palustris
3.2
-
 succinyl-CoA mutant enzyme H29R, at pH 7.5 and 37°C Rhodopseudomonas palustris

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Rhodopseudomonas palustris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.88
-
 glycine mutant enzyme H15K, at pH 7.5 and 37°C Rhodopseudomonas palustris
1.18
-
 glycine wild type enzyme, at pH 7.5 and 37°C Rhodopseudomonas palustris
1.22
-
 glycine mutant enzyme H29R, at pH 7.5 and 37°C Rhodopseudomonas palustris
130
-
 succinyl-CoA wild type enzyme, at pH 7.5 and 37°C Rhodopseudomonas palustris
140
-
 succinyl-CoA mutant enzyme H29R, at pH 7.5 and 37°C Rhodopseudomonas palustris
230
-
 succinyl-CoA mutant enzyme H15K, at pH 7.5 and 37°C Rhodopseudomonas palustris