Cloned (Comment) | Organism |
---|---|
expression of erythroid-specific isoform ALAS-E and mutants in Escherichia coli | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D279A | exchange mutant of potential cofactor binding residue Asp279, no activity, dissociation constant for pyridoxal 5'-phosphate is 19fold increased, different mode of cofactor binding, no formation of quinonoid reaction intermediate, which can be restored by addition of analogue N-methyl-pyridoxal 5'-phosphate | Mus musculus |
D279E | exchange mutant of potential cofactor binding residue Asp279, 30fold reduced catalytic efficiency for succinyl-CoA compared to the wild-type | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
succinyl-CoA | recombinant erythroid isoform wild-type | Mus musculus | |
0.035 | - |
succinyl-CoA | recombinant erythroid isoform mutant D279E | Mus musculus | |
23 | - |
glycine | recombinant erythroid isoform wild-type | Mus musculus | |
140 | - |
glycine | recombinant erythroid isoform mutant D279E | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
erythroid-specific isoform | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutants from E. coli | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 | Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | - |
Mus musculus | 5-aminolevulinate + CoA + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0967 | - |
succinyl-CoA | recombinant erythroid isoform mutant D279E | Mus musculus | |
0.167 | - |
succinyl-CoA | recombinant erythroid isoform | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
N-methyl-pyridoxal 5'-phosphate | in vitro, mutant D279A | Mus musculus | |
pyridoxal 5'-phosphate | required | Mus musculus |