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Literature summary for 2.3.1.37 extracted from

  • Tan, D.; Barber, M.J.; Ferreira, G.C.
    The role of tyrosine 121 in cofactor binding of 5-aminolevulinate synthase (1998), Protein Sci., 7, 1208-1213.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.37

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His-tagged wild-type and mutants in Escherichia coli Mus musculus

Protein Variants

Protein Variants Comment Organism
Y121F exchange mutant of potential cofactor binding residue Tyr121, 5% activity compared to the wild-type, Km for glycine is 5fold increased, lower affinity for pyridoxal 5'phosphate Mus musculus
Y121H exchange mutant of potential cofactor binding residue Tyr121, 36% activity compared to the wild-type, Km for glycine is 34fold increased, lower affinity for pyridoxal 5'phosphate Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 succinyl-CoA recombinant mutant Y121H Mus musculus
0.002
-
 succinyl-CoA recombinant erythroid isoform wild-type Mus musculus
0.014
-
 succinyl-CoA recombinant mutant Y121F Mus musculus
11.7
-
 glycine recombinant erythroid isoform Mus musculus
52.2
-
 glycine recombinant mutant Y121H Mus musculus
400
-
 glycine recombinant mutant Y121F Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid sequence alignment Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 erythroid-specific isoform
-

Purification (Commentary)

Purification (Comment) Organism
recombinant of His-tagged wild-type and mutants from E. coli Mus musculus

Reaction

Reaction Comment Organism Reaction ID
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation Mus musculus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + glycine
-
 Mus musculus 5-aminolevulinate + CoA + CO2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0355
-
 succinyl-CoA recombinant erythroid isoform mutant Y121H Mus musculus
0.235
-
 succinyl-CoA recombinant erythroid isoform mutant Y121F Mus musculus
0.658
-
 succinyl-CoA recombinant erythroid isoform Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate required Mus musculus