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Literature summary for 2.3.1.37 extracted from
- Expression of mammalian 5-aminolevulinate synthase in Escherichia coli. Overproduction, purification, and characterization (1993), J. Biol. Chem., 268, 584-590.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of mouse erythroid enzyme in Escherichia coli | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.055 | - |
succinyl-CoA | recombinant protein | Mus musculus |  |
| 51 | - |
glycine | recombinant protein | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
2 * 56000, mouse enzyme expressed in Escherichia coli, SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinyl-CoA + glycine | Mus musculus | first step in heme biosynthesis pathway | 5-aminolevulinate + CoA + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
erythroid-specific isoform | - |
| Mus musculus | - |
gene ALAS-2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from E. coli | Mus musculus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinyl-CoA + glycine | - |
Mus musculus | 5-aminolevulinate + CoA + CO2 | - |
? | |
| succinyl-CoA + glycine | first step in heme biosynthesis pathway | Mus musculus | 5-aminolevulinate + CoA + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 56000, mouse enzyme expressed in Escherichia coli, SDS-PAGE | Mus musculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0095 | - |
succinyl-CoA | - |
Mus musculus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Mus musculus | |
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