Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | loosely bound | Gallus gallus | 5743 | - |
mitochondrial inner membrane | loosely bound | Rattus norvegicus | 5743 | - |
mitochondrial matrix | - |
Gallus gallus | 5759 | - |
mitochondrial matrix | - |
Rattus norvegicus | 5759 | - |
mitochondrion | - |
Gallus gallus | 5739 | - |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | Gallus gallus | rate-limiting enzyme of heme biosynthesis | 5-aminolevulinate + CoA + CO2 | - |
? | |
succinyl-CoA + glycine | Rattus norvegicus | rate-limiting enzyme of heme biosynthesis | 5-aminolevulinate + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
difficulties due to the tendency of the enzyme to form large MW aggregates and to its susceptibility to proteolytic degradation | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Gallus gallus | - |
embryo | - |
Rattus norvegicus | - |
liver | fetal | Gallus gallus | - |
liver | fetal | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.16 | - |
purified enzyme | Rattus norvegicus |
0.58 | - |
purified enzyme | Gallus gallus |
Storage Stability | Organism |
---|---|
-195°C, following an initial loss of 20% of activity due to freeze-thawing the enzyme loses no further activity for at least 6 months | Gallus gallus |
-195°C, following an initial loss of 20% of activity due to freeze-thawing the enzyme loses no further activity for at least 6 months | Rattus norvegicus |
4°C, 50 mM Tris-HCl, 100 mM glycine, 0.1 mM pyridoxal 5'-phosphate, 1 mM dithioerythritol, pH 7.6, 5 mM 5'-AMP, stable for at least 4 months | Gallus gallus |
4°C, 50 mM Tris-HCl, 100 mM glycine, 0.1 mM pyridoxal 5'-phosphate, 1 mM dithioerythritol, pH 7.6, 5 mM 5'-AMP, stable for at least 4 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | - |
Gallus gallus | 5-aminolevulinate + CoA + CO2 | - |
? | |
succinyl-CoA + glycine | - |
Rattus norvegicus | 5-aminolevulinate + CoA + CO2 | - |
? | |
succinyl-CoA + glycine | rate-limiting enzyme of heme biosynthesis | Gallus gallus | 5-aminolevulinate + CoA + CO2 | - |
? | |
succinyl-CoA + glycine | rate-limiting enzyme of heme biosynthesis | Rattus norvegicus | 5-aminolevulinate + CoA + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Gallus gallus |
37 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Gallus gallus | |
pyridoxal 5'-phosphate | - |
Rattus norvegicus |