Literature summary for 2.3.1.35 extracted from

Hernandez, V.M.; Girard, L.; Hernandez-Lucas, I.; Vazquez, A.; Ortiz-Ortiz, C.; Diaz, R.; Dunn, M.F.
Genetic and biochemical characterization of arginine biosynthesis in Sinorhizobium meliloti 1021 (2015), Microbiology, 161, 1671-1682 .

Search for more literature for 2.3.1.35

Inhibitors

Inhibitors	Comment	Organism	Structure
L-ornithine	-	Sinorhizobium meliloti

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.1	-	N2-Acetyl-L-ornithine	pH 8.0, 30°C	Sinorhizobium meliloti
8.2	-	L-glutamate	pH 8.0, 30°C	Sinorhizobium meliloti

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
88000	-	gel filtration	Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N2-acetyl-L-ornithine + L-glutamate	Sinorhizobium meliloti	-	L-ornithine + N-acetyl-L-glutamate	-	?

Organism

Organism	UniProt	Comment	Textmining
Sinorhizobium meliloti	Q92MJ1	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	autoproteolysis occurs between A194/T195, which results in generation of alpha and beta polypeptides	Sinorhizobium meliloti

Purification (Commentary)

Purification (Comment)	Organism
-	Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N2-acetyl-L-ornithine + L-glutamate	-	Sinorhizobium meliloti	L-ornithine + N-acetyl-L-glutamate	-	?

Subunits

Subunits	Comment	Organism
tetramer	1 * 23000 (alpha) + 2 * 25000 (beta)	Sinorhizobium meliloti

Synonyms

Synonyms	Comment	Organism
argJ	-	Sinorhizobium meliloti

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Sinorhizobium meliloti

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Sinorhizobium meliloti

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
2.4	-	pH 8.0, 30°C	Sinorhizobium meliloti	L-ornithine

General Information

General Information	Comment	Organism
malfunction	inactivation of argJ does not cause arginine auxotrophy or marked growth defects	Sinorhizobium meliloti

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Release 2023.1 (January 2023)