Crystallization (Comment) | Organism |
---|---|
crystals of OAT2 in complex with L-Glu are generated. Optimization of crystallization conditions lead to a 2.7 A resolution structure for OAT2 acylated at Thr-181 and in complex with L-Glu (referred to as the acetyl-OAT2-glutamate complex) | Streptomyces clavuligerus |
Protein Variants | Comment | Organism |
---|---|---|
D150G | autoprocessing to alpha-,beta-subunits: 50% (compared to wild-type 100%), acetyl transferase activity (production of ornithine from N-alpha-acetyl-L-ornithine/L-Glu): 50% (wild-type 100%) | Streptomyces clavuligerus |
DELTA1389 | autoprocessing to alpha-,beta-subunits: 100% (compared to wild-type 100%), acetyl transferase activity (production of ornithine from N-alpha-acetyl-L-ornithine/L-Glu): 40% (wild-type 100%) | Streptomyces clavuligerus |
E260A | autoprocessing to alpha-,beta-subunits: not determined (compared to wild-type 100%), acetyl transferase activity (production of ornithine from N-alpha-acetyl-L-ornithine/L-Glu): 40% (wild-type 100%) | Streptomyces clavuligerus |
K170A | autoprocessing to alpha-,beta-subunits: 0% (compared to wild-type 100%), acetyl transferase activity (production of ornithine from N-alpha-acetyl-L-ornithine/L-Glu): 5% (wild-type 100%) | Streptomyces clavuligerus |
T148A | autoprocessing to alpha-,beta-subunits: 0% (compared to wild-type 100%), acetyl transferase activity (production of ornithine from N-alpha-acetyl-L-ornithine/L-Glu): 2% (wild-type: 100%) | Streptomyces clavuligerus |
T149A | autoprocessing to alpha-,beta-subunits: 80% (compared to wild-type 100%), acetyl transferase activity (production of ornithine from N-alpha-acetyl-L-ornithine/L-Glu): 10% (wild-type: 100%) | Streptomyces clavuligerus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | P0DJQ5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + N-acetyl-L-glutamate | - |
Streptomyces clavuligerus | N2-acetyl-L-ornithine + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OAT2 | - |
Streptomyces clavuligerus |
ornithine acetyl transferase | - |
Streptomyces clavuligerus |