Literature summary for 2.3.1.35 extracted from

Marc, F.; Weigel, P.; Legrain, C.; Almeras, Y.; Santrot, M.; Glansdorff, N.; Sakanyan, V.
Characterization and kinetic mechanism of mono- and bifunctional ornithine acetyltransferases from thermophilic microorganisms (2000), Eur. J. Biochem., 267, 5217-5226.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Geobacillus stearothermophilus
expression in Escherichia coli	Thermotoga neapolitana
expression in Escherichia coli	Methanocaldococcus jannaschii

Inhibitors

Inhibitors	Comment	Organism	Structure
L-ornithine	-	Geobacillus stearothermophilus
L-ornithine	-	Methanocaldococcus jannaschii
L-ornithine	-	Thermotoga neapolitana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.7	-	alpha-N-acetyl-L-ornithine	-	Geobacillus stearothermophilus
8.1	-	alpha-N-acetyl-L-ornithine	-	Thermotoga neapolitana
9.6	-	alpha-N-acetyl-L-ornithine	-	Methanocaldococcus jannaschii
11.3	-	L-glutamate	-	Methanocaldococcus jannaschii
19.2	-	L-glutamate	-	Geobacillus stearothermophilus
27.9	-	L-glutamate	-	Thermotoga neapolitana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N2-acetyl-L-ornithine + L-glutamate	Geobacillus stearothermophilus	first step in arginine biosynthesis	L-ornithine + N-acetyl-L-glutamate	-	?
N2-acetyl-L-ornithine + L-glutamate	Thermotoga neapolitana	first step in arginine biosynthesis	L-ornithine + N-acetyl-L-glutamate	-	?
N2-acetyl-L-ornithine + L-glutamate	Methanocaldococcus jannaschii	first step in arginine biosynthesis	L-ornithine + N-acetyl-L-glutamate	-	?
N2-acetyl-L-ornithine + L-glutamate	Geobacillus stearothermophilus	bifunctional enzyme catalyzes also the fifth step of arginine biosynthesis	L-ornithine + N-acetyl-L-glutamate	-	?
N2-acetyl-L-ornithine + L-glutamate	Thermotoga neapolitana	bifunctional enzyme catalyzes also the fifth step of arginine biosynthesis	L-ornithine + N-acetyl-L-glutamate	-	?

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	-	-
Methanocaldococcus jannaschii	-	-	-
Thermotoga neapolitana	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
27	-	-	Geobacillus stearothermophilus
175	-	-	Methanocaldococcus jannaschii
290	-	-	Thermotoga neapolitana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + L-glutamate	-	Geobacillus stearothermophilus	CoA + N-acetyl-L-glutamate	-	?
acetyl-CoA + L-glutamate	-	Thermotoga neapolitana	CoA + N-acetyl-L-glutamate	-	?
N2-acetyl-L-ornithine + L-glutamate	-	Geobacillus stearothermophilus	L-ornithine + N-acetyl-L-glutamate	-	?
N2-acetyl-L-ornithine + L-glutamate	-	Thermotoga neapolitana	L-ornithine + N-acetyl-L-glutamate	-	?
N2-acetyl-L-ornithine + L-glutamate	-	Methanocaldococcus jannaschii	L-ornithine + N-acetyl-L-glutamate	-	?
N2-acetyl-L-ornithine + L-glutamate	first step in arginine biosynthesis	Geobacillus stearothermophilus	L-ornithine + N-acetyl-L-glutamate	-	?
N2-acetyl-L-ornithine + L-glutamate	first step in arginine biosynthesis	Thermotoga neapolitana	L-ornithine + N-acetyl-L-glutamate	-	?
N2-acetyl-L-ornithine + L-glutamate	first step in arginine biosynthesis	Methanocaldococcus jannaschii	L-ornithine + N-acetyl-L-glutamate	-	?
N2-acetyl-L-ornithine + L-glutamate	bifunctional enzyme catalyzes also the fifth step of arginine biosynthesis	Geobacillus stearothermophilus	L-ornithine + N-acetyl-L-glutamate	-	?
N2-acetyl-L-ornithine + L-glutamate	bifunctional enzyme catalyzes also the fifth step of arginine biosynthesis	Thermotoga neapolitana	L-ornithine + N-acetyl-L-glutamate	-	?

Subunits

Subunits	Comment	Organism
heterotetramer	-	Geobacillus stearothermophilus
heterotetramer	-	Thermotoga neapolitana
heterotetramer	-	Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
75	-	-	Geobacillus stearothermophilus
90	-	-	Thermotoga neapolitana
95	-	more than 95°C	Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Geobacillus stearothermophilus
8	-	-	Thermotoga neapolitana
8	-	-	Methanocaldococcus jannaschii

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	10	-	Geobacillus stearothermophilus
7	10	-	Thermotoga neapolitana
7	10	-	Methanocaldococcus jannaschii

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Release 2023.1 (January 2023)