Cloned (Comment) | Organism |
---|---|
mutant enzymes expressed in Saccharomyces cerevisiae | Schizosaccharomyces pombe |
Protein Variants | Comment | Organism |
---|---|---|
D209N | kcat/Km for acetyl-CoA is 1.45fold lower than wild-type value, kcat/Km for L-homocysteine is 1.6fold higher than wild-type value | Schizosaccharomyces pombe |
D374N | kcat/Km for acetyl-CoA is 1.6fold than wild-type value, kcat/Km for L-homocysteine is 1.6fold higher than wild-type value | Schizosaccharomyces pombe |
D403N | inactive mutant enzyme | Schizosaccharomyces pombe |
H432A | inactive mutant enzyme | Schizosaccharomyces pombe |
S163A | inactive mutant enzyme | Schizosaccharomyces pombe |
S163C | kcat/Km for acetyl-CoA is 16.7fold than wild-type value, kcat/Km for L-homocysteine is 33fold lower than wild-type value | Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0116 | - |
acetyl-CoA | 21°C, pH 8.0, mutant enzyme | Schizosaccharomyces pombe | |
0.0199 | - |
acetyl-CoA | 21°C, pH 8.0, mutant enzyme | Schizosaccharomyces pombe | |
0.0209 | - |
acetyl-CoA | 21°C, pH 8.0, wild-type enzyme | Schizosaccharomyces pombe | |
0.0239 | - |
acetyl-CoA | 21°C, pH 8.0, mutant enzyme | Schizosaccharomyces pombe | |
0.0441 | - |
butyryl-CoA | 21°C, pH 8.0, wild-type enzyme | Schizosaccharomyces pombe | |
0.0484 | - |
propionyl-CoA | 21°C, pH 8.0, wild-type enzyme | Schizosaccharomyces pombe | |
0.96 | - |
L-homoserine | 21°C, pH 8.0, mutant enzyme | Schizosaccharomyces pombe | |
1.09 | - |
L-homoserine | 21°C, pH 8.0, wild-type enzyme | Schizosaccharomyces pombe | |
1.15 | - |
L-homoserine | 21°C, pH 8.0, mutant enzyme | Schizosaccharomyces pombe | |
6.43 | - |
L-homoserine | 21°C, pH 8.0, mutant enzyme | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-homoserine | Schizosaccharomyces pombe | the enzyme is a catalyst in the biochemical pathway that metabolizes Asp to Met in fungi | CoA + O-acetyl-L-homoserine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-homoserine | the enzyme is a catalyst in the biochemical pathway that metabolizes Asp to Met in fungi | Schizosaccharomyces pombe | CoA + O-acetyl-L-homoserine | - |
? | |
acetyl-CoA + L-homoserine | homoserine transacetylase catalyzes the acetylation of L-homoserine via a covalent acyl-enzyme intermediate through an active-site Ser | Schizosaccharomyces pombe | CoA + O-acetyl-L-homoserine | - |
? | |
butyryl-CoA + L-homoserine | - |
Schizosaccharomyces pombe | CoA + O-butyryl-L-homoserine | - |
? | |
propionyl-CoA + L-homoserine | - |
Schizosaccharomyces pombe | CoA + O-propionyl-L-homoserine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | 0.51 | acetyl-CoA | 21°C, pH 8.0, mutant enzyme | Schizosaccharomyces pombe | |
0.64 | - |
acetyl-CoA | 21°C, pH 8.0, mutant enzyme | Schizosaccharomyces pombe | |
1.67 | - |
L-homoserine | 21°C, pH 8.0, mutant enzyme | Schizosaccharomyces pombe | |
8.4 | - |
L-homoserine | 21°C, pH 8.0, mutant enzyme | Schizosaccharomyces pombe | |
8.46 | - |
acetyl-CoA | 21°C, pH 8.0, mutant enzyme | Schizosaccharomyces pombe | |
9.34 | - |
acetyl-CoA | 21°C, pH 8.0, wild-type enzyme | Schizosaccharomyces pombe | |
9.6 | - |
L-homoserine | 21°C, pH 8.0, wild-type enzyme | Schizosaccharomyces pombe | |
12.9 | - |
acetyl-CoA | 21°C, pH 8.0, mutant enzyme | Schizosaccharomyces pombe | |
13.4 | - |
L-homoserine | 21°C, pH 8.0, mutant enzyme | Schizosaccharomyces pombe | |
16.1 | - |
propionyl-CoA | 21°C, pH 8.0, wild-type enzyme | Schizosaccharomyces pombe | |
47.2 | - |
butyryl-CoA | 21°C, pH 8.0, wild-type enzyme | Schizosaccharomyces pombe |