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Literature summary for 2.3.1.31 extracted from
- Partial purification and some properties of homoserine O-acetyltransferase of a methionine auxotroph of Saccharomyces cerevisiae (1987), J. Bacteriol., 169, 3458-3463.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | methionine auxotroph strains show no activity | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DL-Penicillamine | 3.3 mM, complete inhibition | Saccharomyces cerevisiae |  |
| L-cysteine | weak | Saccharomyces cerevisiae | |
| L-homocysteine | - |
Saccharomyces cerevisiae | |
| L-methionine | no inhibition; repression of enzyme expression | Saccharomyces cerevisiae | |
| additional information | no inhibition by MgCl2, MnCl2, ZnCl2, hydroxylamine, phenylhydrazine, D-homoserine, methionine, L-tyrosine, L-serine and nucleotides | Saccharomyces cerevisiae | |
| O-acetyl-L-homoserine | - |
Saccharomyces cerevisiae | |
| O-acetyl-L-serine | weak | Saccharomyces cerevisiae | |
| O-phospho-L-homoserine | weak | Saccharomyces cerevisiae | |
| O-succinyl-L-homoserine | weak | Saccharomyces cerevisiae | |
| S-adenosyl-L-homocysteine | weak | Saccharomyces cerevisiae | |
| S-adenosyl-L-methionine | weak | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
acetyl-CoA | deacylation | Saccharomyces cerevisiae | |
| 0.027 | - |
acetyl-CoA | - |
Saccharomyces cerevisiae | |
| 1 | - |
L-homoserine | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 101000 | 104000 | sucrose density gradient centrifugation, gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
haploid wild-type strain (a-mating type) and met-auxotroph mutant strains 6,8,12,13,15, and 17 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, mutant strain 6 | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 67.4 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + L-homoserine | succinyl-CoA cannot replace acetyl-CoA | Saccharomyces cerevisiae | CoA + O-acetyl-L-homoserine | - |
ir | |
| acetyl-CoA + L-homoserine | not D-homoserine or any other hydroxy-L-amino acid | Saccharomyces cerevisiae | CoA + O-acetyl-L-homoserine | - |
ir | |
| additional information | deacylates acetyl-CoA in the absence of L-homoserine | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | catalyzes reversible L-homoserine/O-acetyl-L-homoserine exchange in the absence of acetyl-CoA | Saccharomyces cerevisiae | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 4.0 | Saccharomyces cerevisiae |
| 7.5 | - |
- |
Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
most stable | Saccharomyces cerevisiae |
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