Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ethionine | - |
Paenibacillus polymyxa | |
L-methionine | addition of S-adenosyl-L-methionine enhances the inhibitory effect; multivalent feed-back inhibition | Paenibacillus polymyxa | |
additional information | no inhibition by Cu2+, Cd2+, Co2+, Fe2+, Hg2+, Mg2+, Mn2+, Ni2+, CoA | Paenibacillus polymyxa | |
S-adenosyl-L-methionine | addition of L-methionine enhances the inhibitory effect; multivalent feed-back inhibition | Paenibacillus polymyxa | |
Zn2+ | reversible | Paenibacillus polymyxa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
acetyl-CoA | - |
Paenibacillus polymyxa | |
0.2 | - |
L-homoserine | - |
Paenibacillus polymyxa | |
10 | - |
L-homoserine | exchange reaction | Paenibacillus polymyxa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
1 * 37000, SDS-PAGE | Paenibacillus polymyxa |
40000 | - |
sucrose density gradient centrifugation, PAGE | Paenibacillus polymyxa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus polymyxa | - |
met-auxotroph strain M4 | - |
Purification (Comment) | Organism |
---|---|
partial | Paenibacillus polymyxa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.17 | - |
- |
Paenibacillus polymyxa |
Storage Stability | Organism |
---|---|
-10°C, t1/2: 3 months | Paenibacillus polymyxa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-homoserine | - |
Paenibacillus polymyxa | CoA + O-acetyl-L-homoserine | - |
? | |
additional information | catalyzes reversible L-homoserine/O-acetyl-L-homoserine exchange in the absence of acetyl-CoA | Paenibacillus polymyxa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, SDS-PAGE | Paenibacillus polymyxa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Paenibacillus polymyxa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
t1/2: 15 min | Paenibacillus polymyxa |
8 | - |
t1/2: 10 min | Paenibacillus polymyxa |
25 | - |
most rapid and irreversible inactivation | Paenibacillus polymyxa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Paenibacillus polymyxa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.6 | 9 | more than half-maximal activity over this range | Paenibacillus polymyxa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
Zn2+ | - |
Paenibacillus polymyxa |