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Literature summary for 2.3.1.305 extracted from

  • Sweet, C.; Williams, A.; Karbarz, M.; Werts, C.; Kalb, S.; Cotter, R.; Raetz, C.
    Enzymatic synthesis of lipid A molecules with four amide-linked acyl chains LpxA acyltransferases selective for an analog of UDP-N-acetylglucosamine in which an amine replaces the 3''-hydroxyl group (2004), J. Biol. Chem., 279, 25411-25419 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.305

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3R)-3-hydroxydecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Mesorhizobium loti
-
 acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxydecanoyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxydecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Acidithiobacillus ferrooxidans
-
 acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxydecanoyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxydecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Mesorhizobium loti ATCC 700743
-
 acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxydecanoyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Mesorhizobium loti
-
 acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxylauroyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Acidithiobacillus ferrooxidans
-
 acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxylauroyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai IPAV
-
 acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxylauroyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Mesorhizobium loti ATCC 700743
-
 acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxylauroyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai IPAV 56601
-
 acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxylauroyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Mesorhizobium loti
-
 acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxymyristoyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Acidithiobacillus ferrooxidans
-
 acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxymyristoyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Mesorhizobium loti ATCC 700743
-
 acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxymyristoyl]amino]-alpha-D-glucopyranose
-
 ?
additional information Acidithiobacillus ferrooxidans enzyme is also able to accept UDP-N-acetyl-alpha-D-glucosamine, cf. EC 2.3.1.129 and displays relaxed acyl donor chain length selectivity ?
-
-
additional information Mesorhizobium loti poor substrate: 3-hydroxypalmitoyl-[acyl-carrier protein] ?
-
-
additional information Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai IPAV poor substrates: (3R)-3-hydroxydecanoyl-[acyl-carrier protein], 3-hydroxypalmitoyl-[acyl-carrier protein], (3R)-3-hydroxymyristoyl-[acyl-carrier protein]. Enzyme is highly specific for 2,3-diamino-2,3-dideoxy-D-glucopyranose ?
-
-
additional information Mesorhizobium loti ATCC 700743 poor substrate: 3-hydroxypalmitoyl-[acyl-carrier protein] ?
-
-
additional information Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai IPAV 56601 poor substrates: (3R)-3-hydroxydecanoyl-[acyl-carrier protein], 3-hydroxypalmitoyl-[acyl-carrier protein], (3R)-3-hydroxymyristoyl-[acyl-carrier protein]. Enzyme is highly specific for 2,3-diamino-2,3-dideoxy-D-glucopyranose ?
-
-

Organism

Organism UniProt Comment Textmining
Acidithiobacillus ferrooxidans Q6QHI7
-
-
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai IPAV Q8EZA6
-
-
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai IPAV 56601 Q8EZA6
-
-
Mesorhizobium loti
-
-
-
Mesorhizobium loti ATCC 700743
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0005
-
 substrate (3R)-3-hydroxypalmitoyl-[acyl-carrier protein], pH 8, 30°C Acidithiobacillus ferrooxidans
0.00052
-
 substrate (3R)-3-hydroxydecanoyl-[acyl-carrier protein], pH 8, 30°C Mesorhizobium loti
0.0006
-
 substrate (3R)-3-hydroxydecanoyl-[acyl-carrier protein], pH 8, 30°C Acidithiobacillus ferrooxidans
0.0008
-
 substrate (3R)-3-hydroxylauroyl-[acyl-carrier protein], pH 8, 30°C Mesorhizobium loti
0.00081
-
 substrate (3R)-3-hydroxylauroyl-[acyl-carrier protein], pH 8, 30°C Acidithiobacillus ferrooxidans
0.0014
-
 substrate (3R)-3-hydroxylauroyl-[acyl-carrier protein], pH 8, 30°C Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai IPAV
0.002
-
 substrate (3R)-3-hydroxymyristoyl-[acyl-carrier protein], pH 8, 30°C Mesorhizobium loti
0.0034
-
 substrate (3R)-3-hydroxymyristoyl-[acyl-carrier protein], pH 8, 30°C Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R)-3-hydroxydecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 Mesorhizobium loti acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxydecanoyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxydecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 Acidithiobacillus ferrooxidans acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxydecanoyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxydecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 Mesorhizobium loti ATCC 700743 acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxydecanoyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 Mesorhizobium loti acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxylauroyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 Acidithiobacillus ferrooxidans acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxylauroyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai IPAV acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxylauroyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 Mesorhizobium loti ATCC 700743 acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxylauroyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai IPAV 56601 acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxylauroyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 Mesorhizobium loti acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxymyristoyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 Acidithiobacillus ferrooxidans acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxymyristoyl]amino]-alpha-D-glucopyranose
-
 ?
(3R)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 Mesorhizobium loti ATCC 700743 acyl-carrier protein + UDP-2-acetamido-2,3-dideoxy-3-[[(3R)-3-hydroxymyristoyl]amino]-alpha-D-glucopyranose
-
 ?
additional information enzyme is also able to accept UDP-N-acetyl-alpha-D-glucosamine, cf. EC 2.3.1.129 and displays relaxed acyl donor chain length selectivity Acidithiobacillus ferrooxidans ?
-
-
additional information poor substrate: 3-hydroxypalmitoyl-[acyl-carrier protein] Mesorhizobium loti ?
-
-
additional information poor substrates: (3R)-3-hydroxydecanoyl-[acyl-carrier protein], 3-hydroxypalmitoyl-[acyl-carrier protein], (3R)-3-hydroxymyristoyl-[acyl-carrier protein]. Enzyme is highly specific for 2,3-diamino-2,3-dideoxy-D-glucopyranose Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai IPAV ?
-
-
additional information poor substrate: 3-hydroxypalmitoyl-[acyl-carrier protein] Mesorhizobium loti ATCC 700743 ?
-
-
additional information poor substrates: (3R)-3-hydroxydecanoyl-[acyl-carrier protein], 3-hydroxypalmitoyl-[acyl-carrier protein], (3R)-3-hydroxymyristoyl-[acyl-carrier protein]. Enzyme is highly specific for 2,3-diamino-2,3-dideoxy-D-glucopyranose Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai IPAV 56601 ?
-
-

Synonyms

Synonyms Comment Organism
LpxA
-
 Mesorhizobium loti
LpxA
-
 Acidithiobacillus ferrooxidans
LpxA
-
 Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai IPAV

General Information

General Information Comment Organism
metabolism enzyme is also able to accept UDP-N-acetyl-alpha-D-glucosamine, cf. EC 2.3.1.129 and displays relaxed acyl donor chain length selectivity Acidithiobacillus ferrooxidans
physiological function this LpxA acyltransferase requires an UDP-GlcNAc derivative in which an amine replaces the glucosamine 3-OH group. The enzyme from Mesorhizobium loti functions almost equally well with 10-, 12-, and 14-carbon 3-hydroxyacyl-[ACP]s. Acylation takes place on the nitrogen atom of UDP-GlcNAc3N at the pyranose 3-position Mesorhizobium loti