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Literature summary for 2.3.1.304 extracted from
- Polyhydroxyalkanoate (PHA) biosynthesis in Thermus thermophilus purification and biochemical properties of PHA synthase (2003), Mol. Cell. Biochem., 254, 173-183 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetyl phosphate | 10 mM acetyl phosphate activates both the native and the dephosphorylated enzyme 6fold and 2fold, respectively | Thermus thermophilus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-ethyl-maleimide | 1 mM N-ethyl-maleimide for 15 min inhibits the enzyme by 56% | Thermus thermophilus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Thermus thermophilus | 5829 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | - |
Thermus thermophilus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-cellulose column chromatography, Cibacron Blue column chromatography, omega-amino octyl-agarose column chromatography, and hydroxyapatite column chromatography | Thermus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.48 | - |
crude extract, at pH 8.5 and 70°C | Thermus thermophilus |
| 2050 | - |
after 4271fold purification, at pH 8.5 and 70°C | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DL-3-beta-hydroxybutyryl-CoA + poly[DL-3-hydroxybutyrate]n | - |
Thermus thermophilus | CoA + poly[DL-3-hydroxybutyrate]n+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE | Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PHA synthase | - |
Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
- |
Thermus thermophilus |
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