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Literature summary for 2.3.1.304 extracted from
- A novel and wide substrate specific polyhydroxyalkanoate (PHA) synthase from unculturable bacteria found in mangrove soil (2018), J. Polym. Res., 25, 23 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the PHA synthase is suitable for the biosynthesis of PHAs that can be used in various biomedical applications due to its ability to incorporate the lipase-degradable monomer sequences of 4-hydroxybutyrate (4HB) and 5-hydroxyvalerate (5HV) | uncultured bacterium |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| BP-M-CPF4, DNA and amino acid sequence determination and analysis, functional assessment by in vivo PHA biosynthesis in a PHA-negative mutant, recombinant expression in the PHA-negative mutant of Cupriavidus necator under control of phaC1 promoter from Cupriavidus necator, subcloning in Escherichia coli | uncultured bacterium |
| expressed in Cupriavidus necator strain PHB-4 | uncultured bacterium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | recombinant phaC-deficient Cupriavidus necator expressing the enzyme is able to accumulate PHA homopolymers and copolymers including poly(3-hydroxybutyrate) [P(3HB)], poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)], poly(3-hydroxybutyrate-co-5-hydroxyvalerate) [P(3HB-co-5HV)], poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)], poly(3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate) [P(3HB-co-3H4MV)], and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)], when suitable precursors are provided | uncultured bacterium |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
uncultured bacterium | 5737 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n | uncultured bacterium | - |
CoA + poly[(R)-3-hydroxyalkanoate]n+1 | - |
? |  |
| additional information | uncultured bacterium | recombinant enzyme, expressed in phaC-deficient Cupriavidus necator, is able to accumulate PHA homopolymers and copolymers including poly(3-hydroxybutyrate) [P(3HB)], poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)], poly(3-hydroxybutyrate-co-5-hydroxyvalerate) [P(3HB-co-5HV)], poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)], poly(3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate) [P(3HB-co-3H4MV)], and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)], when suitable precursors are provided | ? | - |
- |
|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured bacterium | - |
isolated from mangrove soil collected in the mangrove forest at Balik Pulau, Penang, Malaysia | - |
| uncultured bacterium | - |
uncultured bacterium from Balik Pulau (Penang, Malaysia) mangrove soil | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n | - |
uncultured bacterium | CoA + poly[(R)-3-hydroxyalkanoate]n+1 | - |
? | |
| (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxy-4-methylvalerate] | - |
uncultured bacterium | CoA + poly[(R)-3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate] | - |
? | |
| (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxyhexanoate] | - |
uncultured bacterium | CoA + poly[(R)-3-hydroxybutyrate-co-3-hydroxyhexanoate] | - |
? | |
| (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxyvalerate] | - |
uncultured bacterium | CoA + poly[(R)-3-hydroxybutyrate-co-3-hydroxyvalerate] | - |
? | |
| (3R)-3-hydroxybutyryl-CoA + poly[4-hydroxybutyrate] | - |
uncultured bacterium | CoA + poly[(R)-3-hydroxybutyrate-co-4-hydroxybutyrate] | - |
? | |
| (3R)-3-hydroxybutyryl-CoA + poly[5-hydroxyvalerate] | - |
uncultured bacterium | CoA + poly[(R)-3-hydroxybutyrate-co-5-hydroxyvalerate] | - |
? | |
| additional information | recombinant enzyme, expressed in phaC-deficient Cupriavidus necator, is able to accumulate PHA homopolymers and copolymers including poly(3-hydroxybutyrate) [P(3HB)], poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)], poly(3-hydroxybutyrate-co-5-hydroxyvalerate) [P(3HB-co-5HV)], poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)], poly(3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate) [P(3HB-co-3H4MV)], and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)], when suitable precursors are provided | uncultured bacterium | ? | - |
- |
|
| additional information | the polyhydroxyalkanoate (PHA) synthase (PhaC) from a mangrove soil metagenome possesses a very wide substrate specificity. The enzyme shows the ability to incorporate six types of PHA monomers, 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), 3-hydroxy-4-methylvalerate (3H4MV), 5-hydroxyvalerate (5HV) and 3-hydroxyhexanoate (3HHx) in the presence of suitable precursors | uncultured bacterium | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BP-M-CPF4 | - |
uncultured bacterium |
| PHA synthase | - |
uncultured bacterium |
| PhaC | - |
uncultured bacterium |
| polyhydroxyalkanoate synthase | - |
uncultured bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
in vivo assay at | uncultured bacterium |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | PhaC can polymerize high molecular weight hydrophobic PHA chains in the hydrophilic environment of the cell cytoplasm | uncultured bacterium |
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