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Literature summary for 2.3.1.304 extracted from
- Polyhydroxyalkanoates (PHA) production from synthetic waste using Pseudomonas pseudoflava PHA synthase enzyme activity analysis from P. pseudoflava and P. palleronii (2017), Biores. Technol., 234, 99-105 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | replacement of conventional plastics by bioplastics. Polyhydroxyalkanoates (PHA) are bio-polyesters accumulated in cells by a wide range of bacteria. Polyhydroxyalkanoates production from synthetic waste using Pseudomonas palleronii polyhydroxyalkanoate synthase enzyme activity | Hydrogenophaga palleronii |
| synthesis | replacement of conventional plastics by bioplastics. Polyhydroxyalkanoates (PHA) are bio-polyesters accumulated in cells by a wide range of bacteria. Polyhydroxyalkanoates production from synthetic waste using Pseudomonas pseudoflava polyhydroxyalkanoate synthase enzyme activity | Hydrogenophaga pseudoflava |
| synthesis | the enzyme can be used for polyhydroxyalkanoate production from synthetic wastewater | Hydrogenophaga pseudoflava |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n | Hydrogenophaga palleronii | - |
CoA + poly[(R)-3-hydroxyalkanoate]n+1 | - |
? |  |
| (R)-3-hydroxybutanoyl-CoA + [(R)-3-hydroxybutanoate]n | Hydrogenophaga palleronii | - |
[(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
| (R)-3-hydroxybutanoyl-CoA + [(R)-3-hydroxybutanoate]n | Hydrogenophaga pseudoflava | - |
[(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
| (R)-3-hydroxybutanoyl-CoA + [(R)-3-hydroxybutanoate]n | Hydrogenophaga pseudoflava NBRC-102513 | - |
[(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
| additional information | Hydrogenophaga palleronii | the bacteria produce homo-polymer [poly-3-hydroxybutyrate (P3HB)] when only acetate is used as carbon source, and it produces co-polymer [poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV)] by addition of co-substrate propionate. Evaluation of PHA production by Pseudomonas pseudoflava strain NBRC-102513 from wastewater containing diverse volatile atty acids (VFA), common products of various wastewaters. Analysis of the PHA spectrum produced from different carbon sources, NMR study, overview | ? | - |
- |
|
| additional information | Hydrogenophaga pseudoflava | the bacteria produce homo-polymer [poly-3-hydroxybutyrate (P3HB)] when only acetate is used as carbon source, and it produces co-polymer [poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV)] by addition of co-substrate propionate. Evaluation of PHA production by Pseudomonas pseudoflava strain NBRC-102513 from wastewater containing diverse volatile fatty acids (VFA), common products of various wastewaters. Analysis of the PHA spectrum produced from different carbon sources, NMR study, overview. MW and polydispersity index (PDI, Mw/Mn) of the P3HB produced by Pseudomonas pseudoflava is 17.63 kDa and 3.3 respectively. MW and PDI of the co-polymer P(3HB-co-3HV) produced by Pseudomonas pseudoflava is 52.33 kDa and 5.7 respectively. The Mw of the standard P(3HB-co-3HV) is 110 kDa, and PDI is 4.3. Pseudomonas pseudoflava can produce biopolymers with relatively lower dispersity | ? | - |
- |
|
| additional information | Hydrogenophaga pseudoflava NBRC-102513 | the bacteria produce homo-polymer [poly-3-hydroxybutyrate (P3HB)] when only acetate is used as carbon source, and it produces co-polymer [poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV)] by addition of co-substrate propionate. Evaluation of PHA production by Pseudomonas pseudoflava strain NBRC-102513 from wastewater containing diverse volatile fatty acids (VFA), common products of various wastewaters. Analysis of the PHA spectrum produced from different carbon sources, NMR study, overview. MW and polydispersity index (PDI, Mw/Mn) of the P3HB produced by Pseudomonas pseudoflava is 17.63 kDa and 3.3 respectively. MW and PDI of the co-polymer P(3HB-co-3HV) produced by Pseudomonas pseudoflava is 52.33 kDa and 5.7 respectively. The Mw of the standard P(3HB-co-3HV) is 110 kDa, and PDI is 4.3. Pseudomonas pseudoflava can produce biopolymers with relatively lower dispersity | ? | - |
- |
|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hydrogenophaga palleronii | - |
- |
- |
| Hydrogenophaga pseudoflava | - |
- |
- |
| Hydrogenophaga pseudoflava | A0A4P6X2C3 | - |
- |
| Hydrogenophaga pseudoflava NBRC-102513 | - |
- |
- |
| Hydrogenophaga pseudoflava NBRC-102513 | A0A4P6X2C3 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n | - |
Hydrogenophaga palleronii | CoA + poly[(R)-3-hydroxyalkanoate]n+1 | - |
? | |
| (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxybutyrate]n | - |
Hydrogenophaga pseudoflava | CoA + poly[(R)-3-hydroxybutyrate]n+1 | - |
? | |
| (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxybutyrate]n | - |
Hydrogenophaga palleronii | CoA + poly[(R)-3-hydroxybutyrate]n+1 | - |
? | |
| (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxybutyrate]n | - |
Hydrogenophaga pseudoflava NBRC-102513 | CoA + poly[(R)-3-hydroxybutyrate]n+1 | - |
? | |
| (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxyvalerate] | - |
Hydrogenophaga pseudoflava | CoA + poly[(R)-3-hydroxybutyrate-co-3-hydroxyvalerate] | - |
? | |
| (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxyvalerate] | - |
Hydrogenophaga palleronii | CoA + poly[(R)-3-hydroxybutyrate-co-3-hydroxyvalerate] | - |
? | |
| (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxyvalerate] | - |
Hydrogenophaga pseudoflava NBRC-102513 | CoA + poly[(R)-3-hydroxybutyrate-co-3-hydroxyvalerate] | - |
? | |
| (R)-3-hydroxybutanoyl-CoA + [(R)-3-hydroxybutanoate]n | - |
Hydrogenophaga palleronii | [(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
| (R)-3-hydroxybutanoyl-CoA + [(R)-3-hydroxybutanoate]n | - |
Hydrogenophaga pseudoflava | [(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
| (R)-3-hydroxybutanoyl-CoA + [(R)-3-hydroxybutanoate]n | - |
Hydrogenophaga pseudoflava NBRC-102513 | [(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
| additional information | the bacteria produce homo-polymer [poly-3-hydroxybutyrate (P3HB)] when only acetate is used as carbon source, and it produces co-polymer [poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV)] by addition of co-substrate propionate. Evaluation of PHA production by Pseudomonas pseudoflava strain NBRC-102513 from wastewater containing diverse volatile atty acids (VFA), common products of various wastewaters. Analysis of the PHA spectrum produced from different carbon sources, NMR study, overview | Hydrogenophaga palleronii | ? | - |
- |
|
| additional information | the bacteria produce homo-polymer [poly-3-hydroxybutyrate (P3HB)] when only acetate is used as carbon source, and it produces co-polymer [poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV)] by addition of co-substrate propionate. Evaluation of PHA production by Pseudomonas pseudoflava strain NBRC-102513 from wastewater containing diverse volatile fatty acids (VFA), common products of various wastewaters. Analysis of the PHA spectrum produced from different carbon sources, NMR study, overview. MW and polydispersity index (PDI, Mw/Mn) of the P3HB produced by Pseudomonas pseudoflava is 17.63 kDa and 3.3 respectively. MW and PDI of the co-polymer P(3HB-co-3HV) produced by Pseudomonas pseudoflava is 52.33 kDa and 5.7 respectively. The Mw of the standard P(3HB-co-3HV) is 110 kDa, and PDI is 4.3. Pseudomonas pseudoflava can produce biopolymers with relatively lower dispersity | Hydrogenophaga pseudoflava | ? | - |
- |
|
| additional information | the bacteria produce homo-polymer [poly-3-hydroxybutyrate (P3HB)] when only acetate is used as carbon source, and it produces co-polymer [poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV)] by addition of co-substrate propionate. Evaluation of PHA production by Pseudomonas pseudoflava strain NBRC-102513 from wastewater containing diverse volatile fatty acids (VFA), common products of various wastewaters. Analysis of the PHA spectrum produced from different carbon sources, NMR study, overview. MW and polydispersity index (PDI, Mw/Mn) of the P3HB produced by Pseudomonas pseudoflava is 17.63 kDa and 3.3 respectively. MW and PDI of the co-polymer P(3HB-co-3HV) produced by Pseudomonas pseudoflava is 52.33 kDa and 5.7 respectively. The Mw of the standard P(3HB-co-3HV) is 110 kDa, and PDI is 4.3. Pseudomonas pseudoflava can produce biopolymers with relatively lower dispersity | Hydrogenophaga pseudoflava NBRC-102513 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 63000, SDS-PAGE | Hydrogenophaga pseudoflava |
| ? | x * 63000, SDS-PAGE | Hydrogenophaga palleronii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PHA synthase | - |
Hydrogenophaga pseudoflava |
| PHA synthase | - |
Hydrogenophaga palleronii |
| polyhydroxyalkanoate synthase | - |
Hydrogenophaga palleronii |
| polyhydroxyalkanoate synthase | - |
Hydrogenophaga pseudoflava |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
in vivo assay at | Hydrogenophaga palleronii |
| 30 | - |
in vivo assay at | Hydrogenophaga pseudoflava |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.2 | in vivo activity | Hydrogenophaga pseudoflava |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | PHA synthases have been assigned to three classes based on their substrate specificity and subunit composition. The class I PHA synthases are composed of a single type of polypeptide chain and use mainly short chain length hydroxyalkanoic acid CoA thioesters as substrates. The class III PHA synthases are composed by two different subunits, each of approximately 40 kDa. Substrate specificity is the main difference between class II and both class I and class III PHA synthases. Class II PHA synthases integrate specially 3-hydroxyfatty acids of medium chain length (C6-C14) into PHA, and the resulting product is a latex-like polymer. Class I PHA synthases synthesize higher molecular weight PHAs compared with class II PHA synthases | Hydrogenophaga palleronii |
| evolution | PHA synthases have been assigned to three classes based on their substrate specificity and subunit composition. The class I PHA synthases are composed of a single type of polypeptide chain and use mainly short chain length hydroxyalkanoic acid CoA thioesters as substrates. The class III PHA synthases are composed by two different subunits, each of approximately 40 kDa. Substrate specificity is the main difference between class II and both class I and class III PHA synthases. Class II PHA synthases integrate specially 3-hydroxyfatty acids of medium chain length (C6-C14) into PHA, and the resulting product is a latex-like polymer. Class I PHA synthases synthesize higher molecular weight PHAs compared with class II PHA synthases | Hydrogenophaga pseudoflava |
| metabolism | PHA synthase is the critical enzyme in PHA biosynthesis | Hydrogenophaga palleronii |
| metabolism | PHA synthase is the critical enzyme in PHA biosynthesis | Hydrogenophaga pseudoflava |
| physiological function | PHA synthase is the critical enzyme in polyhydroxyalkanoates (PHA) biosynthesis, and R-3-hydroxyacyl-coenzyme A (CoA) is the substrate. Poly-3-hydroxybutyrate (P3HB) is one type of PHA produced by many bacteria | Hydrogenophaga palleronii |
| physiological function | PHA synthase is the critical enzyme in polyhydroxyalkanoates (PHA) biosynthesis, and R-3-hydroxyacyl-coenzyme A (CoA) is the substrate. Poly-3-hydroxybutyrate (P3HB) is one type of PHA produced by many bacteria | Hydrogenophaga pseudoflava |
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