Cloned (Comment) | Organism |
---|---|
expression of PhaRCBm, and as hybrid enzyme PhaRYB4CBm with Bacillus cereus YB-4, PhaRCYB4 in Escherichia coli JM109 | Priestia megaterium |
expression of PhaRCYB4, and as hybrid enzyme PhaRYB4CBm with Bacillus megaterium NBRC15308, PhaRCBm in Escherichia coli JM109 | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of enzyme hybrids of PhaC from Bacillus megaterium and Bacillus cereus, i.e. PhaRBmCYB4 and PhaRYB4CBm. The molecular weight of P(3HB) synthesized by PhaRCYB4 decreased with increasing culture time and temperature, time-dependent behavior is observed for hybrid synthase PhaRBmCYB4, but not for PhaRYB4CBm, thus the molecular weight change is caused by the PhaCYB4 subunit | Priestia megaterium |
additional information | construction of enzyme hybrids of PhaC from Bacillus megaterium and Bacillus cereus, i.e. PhaRBmCYB4 and PhaRYB4CBm. The molecular weight of P(3HB) synthesized by PhaRCYB4 decreased with increasing culture time and temperature, time-dependent behavior is observed for hybrid synthase PhaRBmCYB4, but not for PhaRYB4CBm, thus the molecular weight change is caused by the PhaCYB4 subunit | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n | Bacillus cereus | PhaRCBm synthesizes (R)-3-hydroxybutanoate, P(3HB), with a low-molecular-weight, Mn = 20000 | [(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n | Priestia megaterium | PhaRCBm synthesizes (R)-3-hydroxybutanoate, P(3HB), with a relatively high molecular weight, Mn = 890000 | [(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n | Bacillus cereus YB-4 | PhaRCBm synthesizes (R)-3-hydroxybutanoate, P(3HB), with a low-molecular-weight, Mn = 20000 | [(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n | Priestia megaterium NBRC15308 | PhaRCBm synthesizes (R)-3-hydroxybutanoate, P(3HB), with a relatively high molecular weight, Mn = 890000 | [(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
additional information | Bacillus cereus | the PhaC from BAcillus cereus also shows a polyhydroxybutanoate hydrolyzing activity, time-dependent change in inverse of degree of polymerization during intracellular P(3HB) degradation at a culture temperature of 37°C, overview | ? | - |
? | |
additional information | Bacillus cereus YB-4 | the PhaC from BAcillus cereus also shows a polyhydroxybutanoate hydrolyzing activity, time-dependent change in inverse of degree of polymerization during intracellular P(3HB) degradation at a culture temperature of 37°C, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | D2Z0B8 | isolated as a PHA producer from the polluted soil in Kitakyushu City, Japan | - |
Bacillus cereus YB-4 | D2Z0B8 | isolated as a PHA producer from the polluted soil in Kitakyushu City, Japan | - |
Priestia megaterium | D2Z0B9 | - |
- |
Priestia megaterium NBRC15308 | D2Z0B9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n | - |
Priestia megaterium | [(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n | - |
Bacillus cereus | [(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n | PhaRCBm synthesizes (R)-3-hydroxybutanoate, P(3HB), with a low-molecular-weight, Mn = 20000 | Bacillus cereus | [(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n | PhaRCBm synthesizes (R)-3-hydroxybutanoate, P(3HB), with a relatively high molecular weight, Mn = 890000 | Priestia megaterium | [(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n | - |
Bacillus cereus YB-4 | [(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n | PhaRCBm synthesizes (R)-3-hydroxybutanoate, P(3HB), with a low-molecular-weight, Mn = 20000 | Bacillus cereus YB-4 | [(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n | - |
Priestia megaterium NBRC15308 | [(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n | PhaRCBm synthesizes (R)-3-hydroxybutanoate, P(3HB), with a relatively high molecular weight, Mn = 890000 | Priestia megaterium NBRC15308 | [(R)-3-hydroxybutanoate]n+1 + CoA | - |
? | |
additional information | the PhaC from BAcillus cereus also shows a polyhydroxybutanoate hydrolyzing activity, time-dependent change in inverse of degree of polymerization during intracellular P(3HB) degradation at a culture temperature of 37°C, overview | Bacillus cereus | ? | - |
? | |
additional information | the PhaC from BAcillus cereus also shows a polyhydroxybutanoate hydrolyzing activity, time-dependent change in inverse of degree of polymerization during intracellular P(3HB) degradation at a culture temperature of 37°C, overview | Bacillus cereus YB-4 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
class IV PHA synthase | - |
Priestia megaterium |
class IV PHA synthase | - |
Bacillus cereus |
PhaRCBm | - |
Priestia megaterium |
PhaRCYB4 | - |
Bacillus cereus |