Any feedback?
Literature summary for 2.3.1.302 extracted from
- Spectrophotometric determination of reaction rates and kinetic parameters of a BAHD acyltransferase using DTNB (5,5'-dithio-bis-[2-nitrobenzoic acid]) (2018), Plant Sci., 269, 148-152 .
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | method to measure hydroxycinnamoyl-CoA:5-hydroxyanthranilate N-hydroxycinnamoyltransferase reaction rates in real time using 5,5'-dithio-bis-(2-nitrobenzoic acid) (i.e. DTNB) to spectrophotometrically quantify, in real time, the release of free CoA during the transferase reaction | Trifolium pratense |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Trifolium pratense |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| caffeoyl-CoA | - |
Trifolium pratense |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0074 | - |
caffeoyl-CoA | pH 8, 25°C | Trifolium pratense | |
| 0.34 | - |
L-tyrosine | pH 8, 25°C | Trifolium pratense | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trifolium pratense | A0A384ZRD3 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| caffeoyl-CoA + L-3,4-dihydroxyphenylalanine | - |
Trifolium pratense | ? + CoA | - |
? | |
| feruloyl-CoA + L-phenylalanine | - |
Trifolium pratense | ? + CoA | - |
? | |
| p-coumaroyl-CoA + L-tyrosine | - |
Trifolium pratense | ? + CoA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BAHD acyltransferase | - |
Trifolium pratense |
| HDT1 | - |
Trifolium pratense |
| hydroxycinnamoyl-Coenzyme A hydroxycinnamoyl transferase | - |
Trifolium pratense |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.1 | - |
L-tyrosine | pH 8, 25°C | Trifolium pratense | |
| 10.2 | - |
caffeoyl-CoA | pH 8, 25°C | Trifolium pratense | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.29 | - |
caffeoyl-CoA | pH 8, 25°C | Trifolium pratense | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
