Any feedback?
Literature summary for 2.3.1.300 extracted from
- Characterization of beta-ketoacyl-acyl carrier protein synthase III from Streptomyces glaucescens and its role in initiation of fatty acid biosynthesis (1998), J. Bacteriol., 180, 4481-4486 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptomyces glaucescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| thiolactomycin | 0.02 mM, about 50% inhibition | Streptomyces glaucescens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
isobutanoyl-CoA | pH 7, 30°C | Streptomyces glaucescens | |
| 0.0006 | - |
butanoyl-CoA | pH 7, 30°C | Streptomyces glaucescens | |
| 0.003 | - |
acetyl-CoA | pH 7, 30°C | Streptomyces glaucescens | |
| 0.0037 | - |
malonyl-[acyl-carrier protein] | pH 7, 30°C | Streptomyces glaucescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
gel filtration | Streptomyces glaucescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2S)-2-methylbutanoyl-CoA + malonyl-[acyl-carrier protein] | Streptomyces glaucescens | - |
(4S)-4-methyl-3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2 | - |
? | |
| 2-methylpropanoyl-CoA + malonyl-[acyl-carrier protein] | Streptomyces glaucescens | - |
4-methyl-3-oxopentanoyl-[acyl-carrier-protein] + CoA + CO2 | - |
? | |
| 3-methylbutanoyl-CoA + malonyl-[acyl-carrier protein] | Streptomyces glaucescens | - |
ethyl-3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2 | - |
? | |
| acetyl-CoA + malonyl-[acyl-carrier protein] | Streptomyces glaucescens | - |
3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| butanoyl-CoA + malonyl-[acyl-carrier protein] | Streptomyces glaucescens | - |
3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2 | - |
? | |
| isobutanoyl-CoA + malonyl-[acyl-carrier protein] | Streptomyces glaucescens | - |
5-methyl-3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2 | - |
? | |
| additional information | Streptomyces glaucescens | enzyme demonstrates both beta-ketoacyl-acyl carrier protein synthase activity and acyl coenzyme A:acyl carrier protein transacylase activity in a 1:0.12 ratio | ? | - |
- |
|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces glaucescens | Q54206 | cf. EC 2.3.1.180 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2S)-2-methylbutanoyl-CoA + malonyl-[acyl-carrier protein] | - |
Streptomyces glaucescens | (4S)-4-methyl-3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2 | - |
? | |
| 2-methylpropanoyl-CoA + malonyl-[acyl-carrier protein] | - |
Streptomyces glaucescens | 4-methyl-3-oxopentanoyl-[acyl-carrier-protein] + CoA + CO2 | - |
? | |
| 3-methylbutanoyl-CoA + malonyl-[acyl-carrier protein] | - |
Streptomyces glaucescens | ethyl-3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2 | - |
? | |
| acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Streptomyces glaucescens | 3-oxobutanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| butanoyl-CoA + malonyl-[acyl-carrier protein] | - |
Streptomyces glaucescens | 3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2 | - |
? | |
| isobutanoyl-CoA + malonyl-[acyl-carrier protein] | - |
Streptomyces glaucescens | 5-methyl-3-oxohexanoyl-[acyl-carrier-protein] + CoA + CO2 | - |
? | |
| additional information | enzyme demonstrates both beta-ketoacyl-acyl carrier protein synthase activity and acyl coenzyme A:acyl carrier protein transacylase activity in a 1:0.12 ratio | Streptomyces glaucescens | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 37000, SDS-PAGE | Streptomyces glaucescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-oxoacyl-[acyl-carrier-protein] synthase | - |
Streptomyces glaucescens |
| FabH | - |
Streptomyces glaucescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 7.7 | - |
Streptomyces glaucescens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | FabH is responsible for initiating both straight- and branched-chain fatty acid biosynthesis in Streptomyces glaucescens and the ratio of the various fatty acids produced will be dictated by the ratios of the various acyl-CoA substrates that can react with FabH | Streptomyces glaucescens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
