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Literature summary for 2.3.1.30 extracted from

  • Verma, D.; Antil, M.; Gupta, V.
    Recombinant production of active Streptococcus pneumoniae CysE in E. coli facilitated by codon optimized BL21(DE3)-RIL and detergent (2019), Prep. Biochem. Biotechnol., 49, 368-374 .
    View publication on PubMed
Search for more literature for 2.3.1.30

Cloned(Commentary)

Cloned (Comment) Organism
gene cysE or SP_0589, recombinant expression of His-tagged enzyme in Escherichia coli strain codon optimized strain BL21(DE3)-RIL Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-serine Streptococcus pneumoniae
-
 CoA + O-acetyl-L-serine
-
 ?
acetyl-CoA + L-serine Streptococcus pneumoniae ATCC BAA-334
-
 CoA + O-acetyl-L-serine
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae A0A0H2UNY1
-
-
Streptococcus pneumoniae ATCC BAA-334 A0A0H2UNY1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) and dialysis Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-serine
-
 Streptococcus pneumoniae CoA + O-acetyl-L-serine
-
 ?
acetyl-CoA + L-serine
-
 Streptococcus pneumoniae ATCC BAA-334 CoA + O-acetyl-L-serine
-
 ?

Synonyms

Synonyms Comment Organism
CysE
-
 Streptococcus pneumoniae
SAT
-
 Streptococcus pneumoniae
Spn cysE
-
 Streptococcus pneumoniae
SP_0589
-
 Streptococcus pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Streptococcus pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Streptococcus pneumoniae

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Streptococcus pneumoniae