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Literature summary for 2.3.1.30 extracted from

  • Qiu, J.; Zang, S.; Ma, Y.; Owusu, L.; Zhou, L.; Jiang, T.; Xin, Y.
    Homology modeling and identification of amino acids involved in the catalytic process of Mycobacterium tuberculosis serine acetyltransferase (2017), Mol. Med. Rep., 15, 1343-1347 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene CysE, sequence comparisons, recombinant expression of wild-type and mutant C-terminally His-tagged enzymes in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
D67A site-directed mutagenesis, the mutant shows 9.79% activity compared to wild-type enzyme Mycobacterium tuberculosis
H117A site-directed mutagenesis, the mutant shows 20.82% activity compared to wild-type enzyme Mycobacterium tuberculosis
H82A site-directed mutagenesis, the mutant shows 14.31% activity compared to wild-type enzyme Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-serine Mycobacterium tuberculosis
-
CoA + O-acetyl-L-serine
-
?
acetyl-CoA + L-serine Mycobacterium tuberculosis H37Rv
-
CoA + O-acetyl-L-serine
-
?
acetyl-CoA + L-serine Mycobacterium tuberculosis ATCC 25618
-
CoA + O-acetyl-L-serine
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P95231
-
-
Mycobacterium tuberculosis ATCC 25618 P95231
-
-
Mycobacterium tuberculosis H37Rv P95231
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant His-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-serine
-
Mycobacterium tuberculosis CoA + O-acetyl-L-serine
-
?
acetyl-CoA + L-serine
-
Mycobacterium tuberculosis H37Rv CoA + O-acetyl-L-serine
-
?
acetyl-CoA + L-serine
-
Mycobacterium tuberculosis ATCC 25618 CoA + O-acetyl-L-serine
-
?

Subunits

Subunits Comment Organism
? x * 23770, sequence calculation, x * 30000, recombinant wild-type and mutant enzymes, SDS-PAGE Mycobacterium tuberculosis
More homology modeling of the enzyme's three-dimensional structure using the protein structure 3P47A of the A-chain of Entamoeba histolytica CysE (refined at a resolution of 1.78 A) as template, the CysE protein possesses a left-handed-beta-helix domain, overview Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
CysE
-
Mycobacterium tuberculosis
serine acetyltransferase
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
Mycobacterium tuberculosis

pI Value

Organism Comment pI Value Maximum pI Value
Mycobacterium tuberculosis sequence calculation
-
7.11

General Information

General Information Comment Organism
evolution enzyme CysE belongs to the hexaxadpeptide acetyltransferase family, characterized by imperfect tandem repeats of the hexapeptide motif [LIV]-[GAED]-X2-[STAV]-X Mycobacterium tuberculosis
additional information homology modeling and identification of amino acids involved in the catalytic process. The conserved amino acid residues D67, H82 and H117 may be involved in the catalytic activity and, thus, the function of CysE Mycobacterium tuberculosis