Cloned (Comment) | Organism |
---|---|
gene CysE, sequence comparisons, recombinant expression of wild-type and mutant C-terminally His-tagged enzymes in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
D67A | site-directed mutagenesis, the mutant shows 9.79% activity compared to wild-type enzyme | Mycobacterium tuberculosis |
H117A | site-directed mutagenesis, the mutant shows 20.82% activity compared to wild-type enzyme | Mycobacterium tuberculosis |
H82A | site-directed mutagenesis, the mutant shows 14.31% activity compared to wild-type enzyme | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | Mycobacterium tuberculosis | - |
CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | Mycobacterium tuberculosis H37Rv | - |
CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | Mycobacterium tuberculosis ATCC 25618 | - |
CoA + O-acetyl-L-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P95231 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | P95231 | - |
- |
Mycobacterium tuberculosis H37Rv | P95231 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant His-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | - |
Mycobacterium tuberculosis | CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | - |
Mycobacterium tuberculosis H37Rv | CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | - |
Mycobacterium tuberculosis ATCC 25618 | CoA + O-acetyl-L-serine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23770, sequence calculation, x * 30000, recombinant wild-type and mutant enzymes, SDS-PAGE | Mycobacterium tuberculosis |
More | homology modeling of the enzyme's three-dimensional structure using the protein structure 3P47A of the A-chain of Entamoeba histolytica CysE (refined at a resolution of 1.78 A) as template, the CysE protein possesses a left-handed-beta-helix domain, overview | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
CysE | - |
Mycobacterium tuberculosis |
serine acetyltransferase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Mycobacterium tuberculosis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mycobacterium tuberculosis | sequence calculation | - |
7.11 |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme CysE belongs to the hexaxadpeptide acetyltransferase family, characterized by imperfect tandem repeats of the hexapeptide motif [LIV]-[GAED]-X2-[STAV]-X | Mycobacterium tuberculosis |
additional information | homology modeling and identification of amino acids involved in the catalytic process. The conserved amino acid residues D67, H82 and H117 may be involved in the catalytic activity and, thus, the function of CysE | Mycobacterium tuberculosis |