Cloned (Comment) | Organism |
---|---|
gene dcsE, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Streptomyces lavendulae |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and mutant G52A-P55G enzymes, sitting drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml of protein in 20 mM Tris-HCl, pH 7.6, 0.2 M NaCl, 5 mM dithiothreitol, and 1 mM EDTA, with 0.001 ml of precipitant solution containing 0.1 M Tris-HCl, pH 7.5, 25% w/v PEG 4000, and 0.2 M ammonium acetate, 3 weeks, structure modeling, X-ray diffraction structure determination and analysis at 1.7-1.8 A resolution | Streptomyces lavendulae |
Protein Variants | Comment | Organism |
---|---|---|
G52A | site-directed mutagenesis, no activity with L-serine, but with L-homoserine | Streptomyces lavendulae |
G52A/P55G | site-directed mutagenesis, no activity with L-serine, but with L-homoserine | Streptomyces lavendulae |
P55G | site-directed mutagenesis, increased activity with L-serine and with L-homoserine compared to the wild-type enzyme | Streptomyces lavendulae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
L-serine | recombinant His-tagged mutant P55G enzyme, pH 8.0, 30°C | Streptomyces lavendulae | |
4.9 | - |
L-serine | recombinant His-tagged wild-type enzyme, pH 8.0, 30°C | Streptomyces lavendulae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | Streptomyces lavendulae | - |
CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | Streptomyces lavendulae ATCC 11924 | - |
CoA + O-acetyl-L-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lavendulae | D2Z028 | gene dcsE; gene dcsE | - |
Streptomyces lavendulae ATCC 11924 | D2Z028 | gene dcsE; gene dcsE | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, dialysis and ultrafiltration | Streptomyces lavendulae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | - |
Streptomyces lavendulae | CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | - |
Streptomyces lavendulae ATCC 11924 | CoA + O-acetyl-L-serine | - |
? | |
additional information | the enzyme prefers L-serine as a substrate, but is also active with L-homoserine, EC 2.3.1.31, substrate specificity, overview | Streptomyces lavendulae | ? | - |
? | |
additional information | the enzyme prefers L-serine as a substrate, but is also active with L-homoserine, EC 2.3.1.31, substrate specificity, overview | Streptomyces lavendulae ATCC 11924 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DcsE | - |
Streptomyces lavendulae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces lavendulae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.62 | - |
L-serine | recombinant His-tagged mutant P55G enzyme, pH 8.0, 30°C | Streptomyces lavendulae | |
1.58 | - |
L-serine | recombinant His-tagged wild-type enzyme, pH 8.0, 30°C | Streptomyces lavendulae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces lavendulae |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme displays a high sequence homology to L-homoserine O-acetyltransferase, but it prefers L-serine over L-homoserine as the substrate, structure analysis and modeling, overview | Streptomyces lavendulae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
L-serine | recombinant His-tagged wild-type enzyme, pH 8.0, 30°C | Streptomyces lavendulae | |
1.23 | - |
L-serine | recombinant His-tagged mutant P55G enzyme, pH 8.0, 30°C | Streptomyces lavendulae |