Cloned (Comment) | Organism |
---|---|
isozyme VvSERAT1-1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of GFP-tagged VvSERAT isozyme in Arabidopsis thaliana and in Vitis vinifera protoplasts with specific subcellular localization of the isozyme | Vitis vinifera |
isozyme VvSERAT2-1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of GFP-tagged VvSERAT isozyme in Arabidopsis thaliana and in Vitis vinifera protoplasts with specific subcellular localization of the isozyme | Vitis vinifera |
isozyme VvSERAT2-2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of GFP-tagged VvSERAT isozyme in Arabidopsis thaliana and in Vitis vinifera protoplasts with specific subcellular localization of the isozyme | Vitis vinifera |
isozyme VvSERAT3-1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of GFP-tagged VvSERAT isozyme in Arabidopsis thaliana and in Vitis vinifera protoplasts with specific subcellular localization of the isozyme | Vitis vinifera |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-cysteine | feedback inhibition; feedback inhibition; feedback inhibition; feedback inhibition, isozyme VvSERAT2-1 is almost insensitive to cysteine inhibition | Vitis vinifera |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | VvSERAT2-1, mainly | Vitis vinifera | 9507 | - |
cytosol | - |
Vitis vinifera | 5829 | - |
cytosol | isozyme VvSERAT1-1 | Vitis vinifera | 5829 | - |
cytosol | isozyme VvSERAT3-1 | Vitis vinifera | 5829 | - |
mitochondrion | isozyme VvSERAT2-2, mainly | Vitis vinifera | 5739 | - |
additional information | isozyme subcellular localization and expression analysis | Vitis vinifera | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
294600 | - |
x * 294600, sequence calculation | Vitis vinifera |
323400 | - |
x * 323400, sequence calculation | Vitis vinifera |
323800 | - |
x * 323800, sequence calculation | Vitis vinifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | Vitis vinifera | - |
CoA + O-acetyl-L-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vitis vinifera | - |
cv. Touriga Nacional, isozyme VvSERAT1-1 | - |
Vitis vinifera | A0A0C5DAX6 | isozyme VvSERAT2-2; cv. Touriga Nacional, isozyme VvSERAT2-2 | - |
Vitis vinifera | A0A0C5DES6 | isozyme VvSERAT3-1; cv. Touriga Nacional, isozyme VvSERAT3-1 | - |
Vitis vinifera | B0LLV2 | cv. Touriga Nacional, isozyme VvSERAT2-1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
recombinant enzyme, isozyme VvSERAT2-2 in the cysteine synthase complex, pH and temperature not specified in the publication | Vitis vinifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | - |
Vitis vinifera | CoA + O-acetyl-L-serine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 294600, sequence calculation | Vitis vinifera |
? | x * 323400, sequence calculation | Vitis vinifera |
? | x * 323800, sequence calculation | Vitis vinifera |
Synonyms | Comment | Organism |
---|---|---|
SERAT | - |
Vitis vinifera |
serine acetyltransferase | - |
Vitis vinifera |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.16 | - |
isozyme VvSERAT2-2, pH and temperature not specified in the publication | Vitis vinifera | L-cysteine | |
1.9 | - |
isozyme VvSERAT2-1, pH and temperature not specified in the publication | Vitis vinifera | L-cysteine |
Organism | Comment | Expression |
---|---|---|
Vitis vinifera | the isozyme expression of VvSERAT1-1 is not affected by sufate depletion | additional information |
Vitis vinifera | the isozyme expression of VvSERAT2-2 is not affected by sufate depletion | additional information |
Vitis vinifera | the isozyme expression of VvSERAT3-1 is not affected by sufate depletion | additional information |
Vitis vinifera | isoform-specific induction of VvSERAT2-1 in leaves upon drought,whereas highlight- or temperature-stress hardly trigger VvSERAT2-1 transcription. Prolonged sulfate depletion, which is accompanied with decreased cysteine levels, causes 3.9-4.5fold increase of VvSERAT2-1 mRNA steady state levels at day five and seven, respectively | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the VvSERAT protein family, the identified four members of the VvSERAT protein family are assigned to three distinct groups upon their sequence similarities to Arabidopsis SERATs | Vitis vinifera |
metabolism | serine acetyltransferase provides O-acetylserine for the biosynthesis of cysteine and thereby regulates the activity of O-acetylserine(thiol)lyase, EC 2.5.1.47, which catalyzes the last step of cysteine biosynthesis and reversibly interacts with serine acetyltransferase in the cysteine synthase complex | Vitis vinifera |
metabolism | serine acetyltransferase provides O-acetylserine for the biosynthesis of cysteine and thereby regulates the activity of O-acetylserine(thiol)lyase, EC 2.5.1.47, which catalyzes the last step of cysteine biosynthesis and reversibly interacts with serine acetyltransferase in the cysteine synthase complex. Only isozyme VvSERAT2-1 lacks the canonical C-terminal tail of plant SERATs and does not form the cysteine synthase complex and is almost insensitive to cysteine inhibition | Vitis vinifera |
additional information | the enzyme reversibly interacts with O-acetylserine(thiol)lyase in the cysteine synthase complex | Vitis vinifera |