Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
recombinant enzyme, crystals grew from 0.1 M MES, pH 6.6, 1ß% 2-methyl-2,3-pentanediol, 0.5 M sodium thiocyanate, 5.5 mM cysteine, and SeMet SAT (10 mg/ml) sitting drops, at 20°C within 7 days. 2.2 A crystal structure of the enzyme, which is s dimer of trimers in complex with cysteine | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cysteine | competitive, binds at the serine substrate site, negatively regulates its own synthesis | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A9D4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | - |
Escherichia coli | CoA + O-acetyl-L-serine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SAT | - |
Escherichia coli |