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Literature summary for 2.3.1.30 extracted from

  • Wirtz, M.; Berkowitz, O.; Droux, M.; Hell, R.
    The cysteine synthase complex from plants: mitochondrial serine acetyltransferase from Arabidopsis thaliana carries a bifunctional domain for catalysis and protein-protein interaction (2001), Eur. J. Biochem., 268, 686-693.
    View publication on PubMed
Search for more literature for 2.3.1.30

Cloned(Commentary)

Cloned (Comment) Organism
construction of an inactivated mutant by targeted disruption of CysE gene for usage in complementation assays Escherichia coli
expression in Escherichia coli SAT inactivated mutant Arabidopsis thaliana
expression of wild-type and mutants in Escherichia coli SAT inactivated mutant as S-tagged protein Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
E282A site-directed mutagenesis in C-terminal hexapeptide-repeat domain, enhanced activity, complementation of inactivated Escherichia coli mutant Arabidopsis thaliana
G354A site-directed mutagenesis in C-terminal hexapeptide-repeat domain, reduced activity, partial complementation of inactivated Escherichia coli mutant Arabidopsis thaliana
H309A site-directed mutagenesis in C-terminal hexapeptide-repeat domain, strongly reduced activity Arabidopsis thaliana
H327A site-directed mutagenesis in C-terminal hexapeptide-repeat domain, nearly no activity Arabidopsis thaliana
V353E site-directed mutagenesis in C-terminal hexapeptide-repeat domain, nearly no activity Arabidopsis thaliana
V353E strain BB1 Salmonella enterica subsp. enterica serovar Typhimurium
V353E mutant enzyme does not bind O-acetylserine (thiol) lyase Arabidopsis thaliana
V353E mutant enzyme does not bind O-acetylserine (thiol) lyase Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.145
-
 acetyl-CoA recombinant isozyme A, wild-type Arabidopsis thaliana
0.176
-
 acetyl-CoA recombinant isozyme A, mutant G354A Arabidopsis thaliana
2.4
-
 L-serine recombinant isozyme A, wild-type Arabidopsis thaliana
25.55
-
 L-serine recombinant isozyme A, mutant G354A Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid sequence alignment Arabidopsis thaliana
additional information
-
 amino acid sequence alignment Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-serine Escherichia coli
-
 CoA + O-acetyl-L-serine
-
 ?
acetyl-CoA + L-serine Arabidopsis thaliana
-
 CoA + O-acetyl-L-serine
-
 ?
acetyl-CoA + L-serine Salmonella enterica subsp. enterica serovar Typhimurium
-
 CoA + O-acetyl-L-serine
-
 ?
acetyl-CoA + L-serine Arabidopsis thaliana rate-limiting step of cysteine biosynthesis CoA + O-acetyl-L-serine
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q39218 gene SAT1-6, mitochondrial isozyme
-
Arabidopsis thaliana Q39218 gene SAT-1, isoform A
-
Arabidopsis thaliana Q42538 gene SAT52, isoform C
-
Arabidopsis thaliana Q42588 gene SAT5, isoform B
-
Escherichia coli
-
 EC1801, gene CysE
-
Salmonella enterica subsp. enterica serovar Typhimurium P29847 gene CysE
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutants as S-tagged protein from E. coli Arabidopsis thaliana

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + L-serine = CoA + O-acetyl-L-serine a C-terminal hexapeptide-repeat domain is common for Arabidopsis thaliana isoforms and several other enzyme DNA sequences, it has a catalytic bifunctionality as serine acetyltransferase and in interaction with O-acetylserine (thiol) lyase and is involved in cysteine biosynthesis regulation, computational modeling Arabidopsis thaliana
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00036
-
 purified recombinant mitochondrial isozyme A, mutant H327A Arabidopsis thaliana
0.00134
-
 purified recombinant mitochondrial isozyme A, mutant V353E Arabidopsis thaliana
0.00513
-
 purified recombinant mitochondrial isozyme A, mutant G354A Arabidopsis thaliana
0.032
-
 purified recombinant mitochondrial isozyme A, wild-type Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-serine
-
 Escherichia coli CoA + O-acetyl-L-serine
-
 ?
acetyl-CoA + L-serine
-
 Arabidopsis thaliana CoA + O-acetyl-L-serine
-
 ?
acetyl-CoA + L-serine
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + O-acetyl-L-serine
-
 ?
acetyl-CoA + L-serine rate-limiting step of cysteine biosynthesis Arabidopsis thaliana CoA + O-acetyl-L-serine
-
 ?

Synonyms

Synonyms Comment Organism
More serine acetyltransferase and O-acetylserine (thiol) lyase form the cysteine synthase complex Arabidopsis thaliana
SAT
-
 Escherichia coli
SAT
-
 Arabidopsis thaliana
SAT
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli
25
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli
7.5
-
 assay at Arabidopsis thaliana