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Literature summary for 2.3.1.30 extracted from

  • Takagi, H.; Kobayashi, C.; Kobayashi, S.I.; Nakamori, S.
    PCR random mutagenesis into Escherichia coli serine acetyltransferase: isolation of the mutant enzymes that cause overproduction of L-cysteine and L-cystine due to the desensitization to feedback inhibition (1999), FEBS Lett., 452, 323-327.
    View publication on PubMed
Search for more literature for 2.3.1.30

Cloned(Commentary)

Cloned (Comment) Organism
construction of cysE gene mutants by random PCR mutagenesis, expression in Escherichia coli cysteine auxotrophic strain JM39-8 Escherichia coli

Protein Variants

Protein Variants Comment Organism
E166G/M201V random PCR mutagenesis, cysE mutant, more insensitive to inhibition by L-cysteine than the wild-type, reduced enzyme activity Escherichia coli
M201R random PCR mutagenesis, cysE mutant, more insensitive to inhibition by L-cysteine than the wild-type, reduced enzyme activity Escherichia coli
M201T random PCR mutagenesis, cysE mutant, more insensitive to inhibition by L-cysteine than the wild-type, reduced enzyme activity Escherichia coli
M256A random PCR mutagenesis, cysE mutant, more insensitive to inhibition by L-cysteine than the wild-type, reduced enzyme activity Escherichia coli
additional information e cysteine and cystine production level of mutants Escherichia coli
N51K/R91H/H233Y random PCR mutagenesis, cysE mutant, more insensitive to inhibition by L-cysteine than the wild-type, reduced enzyme activity Escherichia coli
P252R random PCR mutagenesis, cysE mutant, more insensitive to inhibition by L-cysteine than the wild-type, reduced enzyme activity Escherichia coli
S253L random PCR mutagenesis, cysE mutant, more insensitive to inhibition by L-cysteine than the wild-type, reduced enzyme activity Escherichia coli
T167K random PCR mutagenesis, cysE mutant, more insensitive to inhibition by L-cysteine than the wild-type, reduced enzyme activity Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-serine Escherichia coli
-
 CoA + O-acetyl-L-serine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-serine
-
 Escherichia coli CoA + O-acetyl-L-serine
-
 ?