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Literature summary for 2.3.1.30 extracted from

  • Inoue, K.; Noji, M.; Saito, K.
    Determination of the sites required for the allosteric inhibition of serine acetyltransferase by L-cysteine in plants (1999), Eur. J. Biochem., 266, 220-227.
    View publication on PubMed
Search for more literature for 2.3.1.30

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutants in Escherichia coli Arabidopsis thaliana
expression of wild-type and mutants in Escherichia coli Citrullus lanatus subsp. vulgaris

Protein Variants

Protein Variants Comment Organism
E278L site-directed mutagenesis, about 1.7fold more sensitive to inhibition by L-cysteine Citrullus lanatus subsp. vulgaris
F285Y site-directed mutagenesis, about 1.7fold less sensitive to inhibition by L-cysteine Citrullus lanatus subsp. vulgaris
G277C site-directed mutagenesis, about 27fold less sensitive to inhibition by L-cysteine Citrullus lanatus subsp. vulgaris
H282Q site-directed mutagenesis, about 3.5fold less sensitive to inhibition by L-cysteine Citrullus lanatus subsp. vulgaris
M280I site-directed mutagenesis, insensitive to inhibition by L-cysteine Citrullus lanatus subsp. vulgaris
S279T site-directed mutagenesis, as sensitive to inhibition by L-cysteine as the wild-type Citrullus lanatus subsp. vulgaris

Inhibitors

Inhibitors Comment Organism Structure
L-cysteine
-
 Arabidopsis thaliana
L-cysteine recombinant wild-type and mutants, not mutant M280I, inhibitor binding motif Citrullus lanatus subsp. vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Arabidopsis thaliana
additional information
-
 additional information Km of mutant enzymes Citrullus lanatus subsp. vulgaris
0.13
-
 acetyl-CoA
-
 Arabidopsis thaliana
0.13
-
 acetyl-CoA
-
 Citrullus lanatus subsp. vulgaris
0.59
-
 L-serine
-
 Arabidopsis thaliana
0.59
-
 L-serine
-
 Citrullus lanatus subsp. vulgaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-serine Arabidopsis thaliana
-
 CoA + O-acetyl-L-serine
-
 ?
acetyl-CoA + L-serine Citrullus lanatus subsp. vulgaris
-
 CoA + O-acetyl-L-serine
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Citrullus lanatus subsp. vulgaris
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-serine
-
 Arabidopsis thaliana CoA + O-acetyl-L-serine
-
 ?
acetyl-CoA + L-serine
-
 Citrullus lanatus subsp. vulgaris CoA + O-acetyl-L-serine
-
 ?