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Literature summary for 2.3.1.299 extracted from
- A three-step assay for ceramide synthase activity using a fluorescent substrate and HPLC (2015), Lipids, 50, 101-109 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CERS1, recombinant expression in HEK-293 cells | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrosphingosine + palmitoyl-CoA | Homo sapiens | low activity | N-palmitoyldihydrosphingosine + CoA | - |
? | |
| dihydrosphingosine + stearoyl-CoA | Homo sapiens | highly preferred substrate | N-stearoyldihydrosphingosine + CoA | - |
? | |
| additional information | Homo sapiens | clear preference for the C18:0 NBD-dihydroceramide synthesis is observed with extracts of human frontal cortex grey matter | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P27545 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| neuron | CERS1 is highly expressed in neurons | Homo sapiens | - |
| U-87MG cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrosphingosine + palmitoyl-CoA | low activity | Homo sapiens | N-palmitoyldihydrosphingosine + CoA | - |
? | |
| dihydrosphingosine + stearoyl-CoA | highly preferred substrate | Homo sapiens | N-stearoyldihydrosphingosine + CoA | - |
? | |
| additional information | clear preference for the C18:0 NBD-dihydroceramide synthesis is observed with extracts of human frontal cortex grey matter | Homo sapiens | ? | - |
? | |
| additional information | CERS1-expressing HEK293 cell extracts show a clear preference for utilisation of the C18:0 fatty acid substrate when presented with equimolar concentrations of C16:0, C18:0, C24:0, and C24:1 fatty acid substrates. CERS1 preferentially catalyses the addition of 18-carbon (C18:0) fatty acids to dihydrosphingosine. No activity with lignoceroyl-CA, and poor activity with nervonoyl-CoA. Improvement of a fluorescent assay, using HPLC using C16:0, C18:0, and C24:1 fatty acids and commercially available sphinganine-NBD, i.e. 7-nitro-2-1,3-benzoxadiazole-labelled dihydrosphingosine or (2S,3R)-2-amino-18((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)octadecane-1-3-diol, as substrates, very accurate and sensitive method for quantification of CERS activity, method evaluation | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ceramide synthase 1 | - |
Homo sapiens |
| CerS1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | C18 ceramide synthesized by CERS1 is implicated in apoptosis and lethal autophagy | Homo sapiens |
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Release 2023.1 (January 2023)
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