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Literature summary for 2.3.1.299 extracted from

  • Merrill, A.H.; van Echten, G.; Wang, E.; Sandhoff, K.
    Fumonisin B1 inhibits sphingosine (sphinganine) N-acyltransferase and de novo sphingolipid biosynthesis in cultured neurons in situ (1993), J. Biol. Chem., 268, 27299-27306 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
fumonisin B1 reversible competitive inhibition, inhibits ceramide synthase in mouse brain microsomes with a competitive-like kinetic behavior with respect to both sphinganine and stearoyl-CoA. Fumonisin B1 inhibits ceramide synthase activity when palmitoyl-CoA, stearoyl-CoA, or lignoceroyl-CoA are used as the co-substrate, cf. EC 2.3.1.297 and 2.3.1.24 Mus musculus
additional information fumonisins block sphingosine biosynthesis by inhibiting the conversion of sphinganine to dihydroceramides, which precedes introduction of the 4,5-trans-double bond of sphingosine. Fumonisins, mycotoxins produced by Fusarium moniliforme and a number of other fungi, cause neuronal degeneration, liver and renal toxicity, cancer, and other injury to animals. Fumonisin Bl inhibits complex sphirqolipid synthesis from galactose and sphinganine, effects on the amounts of free sphingosine and sphinganine and on total complex sphingolipids, overview Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
stearoyl-CoA pH 7.4, 25°C Mus musculus
0.035
-
D-erythro-sphinganine pH 7.4, 25°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Mus musculus
-
-

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-
Mus musculus NMRI
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Mus musculus
-
cerebellum
-
Mus musculus
-
granule cell
-
Mus musculus
-
neuron cerebellar Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-erythro-sphinganine + stearoyl-CoA
-
Mus musculus N-stearoyl-D-sphinganine + CoA
-
?
D-erythro-sphinganine + stearoyl-CoA
-
Mus musculus NMRI N-stearoyl-D-sphinganine + CoA
-
?

Synonyms

Synonyms Comment Organism
ceramide synthase
-
Mus musculus
More see also EC 2.3.1.24 and EC 2.3.1.297 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics Mus musculus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000075
-
pH 7.4, 25°C Mus musculus fumonisin B1