Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cisplatin | - |
Homo sapiens | |
dithiothreitol | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene CERS1, ectopic expression of N-terminally FLAG3-tagged CerS1 in HEK-293 or HeLa cells, recombinant expression of enzyme mutant H182A/H183A | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
H182A/H183A | site-directed mutagenesis | Mus musculus |
H182A/H183A | the mutant shows greatly reduced activity compared to the wild type enzyme | Homo sapiens |
additional information | in HEK-293 cells, both endogenous human CerS1 and ectopically expressed murine CerS1 have rapid basal turnover, CerS1 proteasomal degradation is ubiquitin mediated. The turnover requires CerS1 activity and is regulated by the opposing actions of p38 MAP kinase and protein kinase C (PKC), p38 MAP kinase is a positive regulator of turnover, while PKC is a negative regulator of turnover. Recombinantly expressed murine isozyme CerS1 sensitizes cells to a wide range of drugs including cisplatin, carboplatin, doxorubicin and vincristine, incontrast to isozymes CerS4 and CerS5. The specific effect of CerS1 is mediated through the activation of the MAP kinase p38 | Mus musculus |
additional information | in HEK-293 cells, both endogenous human CerS1 and ectopically expressed murine CerS1 have rapid basal turnover, the turnover requires CerS1 activity and is regulated by the opposing actions of p38 MAP kinase and protein kinase C (PKC), p38 MAP kinase is a positive regulator of turnover, while PKC is a negative regulator of turnover. Recombinant isozyme CerS1 sensitizes cells to a wide range of drugs including cisplatin, carboplatin, doxorubicin and vincristine, in contrast to isozymes CerS4 and CerS5. The specific effect of CerS1 is mediated through the activation of the MAP kinase p38 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MG132 | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Mus musculus | |
Mg2+ | activates | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphinganine + stearoyl-CoA | Mus musculus | - |
N-stearoylsphinganine + CoA | - |
? | |
sphinganine + stearoyl-CoA | Homo sapiens | - |
N-stearoylsphinganine + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | P27544 | - |
- |
Mus musculus | P27545 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | CerS1 is phosphorylated in vivo and activation of protein kinase C increases the phosphorylation of the protein | Mus musculus |
phosphoprotein | CerS1 is phosphorylated in vivo and activation of protein kinase C increases the phosphorylation of the protein | Homo sapiens |
phosphoprotein | the enzyme turnover is regulated by the opposing actions of p38 MAP kinase and protein kinase C (PKC). p38 MAP kinase is a positive regulator of turnover, while PKC is a negative regulator of turnover. The enzyme is phosphorylated in vivo and activation of PKC increases the phosphorylation of the protein | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
HEK-293 cell | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
U-373MG cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphinganine + stearoyl-CoA | - |
Mus musculus | N-stearoylsphinganine + CoA | - |
? | |
sphinganine + stearoyl-CoA | - |
Homo sapiens | N-stearoylsphinganine + CoA | - |
? | |
stearoyl-CoA + sphinganine | - |
Homo sapiens | N-(stearoyl)-sphinganine + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, FLAG-tagged enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ceramide synthase 1 | - |
Homo sapiens |
ceramide synthase 1 | - |
Mus musculus |
CerS1 | - |
Homo sapiens |
CerS1 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
7.5 | - |
assay at | Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Mus musculus | diverse stresses including chemotherapeutic drugs, UV light and DTT can induce CerS1 turnover | up |
Homo sapiens | diverse stresses including chemotherapeutic drugs, UV light and DTT can induce CerS1 turnover | up |
General Information | Comment | Organism |
---|---|---|
metabolism | in HEK-293 cells, both endogenous human CerS1 and ectopically expressed murine CerS1 have rapid basal turnover, the turnover requires CerS1 activity and is regulated by the opposing actions of p38 MAP kinase and protein kinase C (PKC), p38 MAP kinase is a positive regulator of turnover, while PKC is a negative regulator of turnover | Mus musculus |
metabolism | in HEK-293 cells, both endogenous human CerS1 and ectopically expressed murine CerS1 have rapid basal turnover, the turnover requires CerS1 activity and is regulated by the opposing actions of p38 MAP kinase and protein kinase C (PKC), p38 MAP kinase is a positive regulator of turnover, while PKC is a negative regulator of turnover | Homo sapiens |
physiological function | ceramide synthase 1 (CerS1) is the most structurally and functionally distinct from the other CerS enzymes, the enzyme is regulated via a regulatory mechanism that specifically controls the level of CerS1 via ubiquitination and proteasome dependent protein turnover | Mus musculus |
physiological function | ceramide synthase 1 (CerS1) is the most structurally and functionally distinct from the other CerS enzymes, the enzyme is regulated via a regulatory mechanism that specifically controls the level of CerS1 via ubiquitination and proteasome dependent protein turnover | Homo sapiens |