Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.299 extracted from

  • Sridevi, P.; Alexander, H.; Laviad, E.L.; Pewzner-Jung, Y.; Hannink, M.; Futerman, A.H.; Alexander, S.
    Ceramide synthase 1 is regulated by proteasomal mediated turnover (2009), Biochim. Biophys. Acta, 1793, 1218-1227 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
cisplatin
-
Homo sapiens
dithiothreitol
-
Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene CERS1, ectopic expression of N-terminally FLAG3-tagged CerS1 in HEK-293 or HeLa cells, recombinant expression of enzyme mutant H182A/H183A Mus musculus

Protein Variants

Protein Variants Comment Organism
H182A/H183A site-directed mutagenesis Mus musculus
H182A/H183A the mutant shows greatly reduced activity compared to the wild type enzyme Homo sapiens
additional information in HEK-293 cells, both endogenous human CerS1 and ectopically expressed murine CerS1 have rapid basal turnover, CerS1 proteasomal degradation is ubiquitin mediated. The turnover requires CerS1 activity and is regulated by the opposing actions of p38 MAP kinase and protein kinase C (PKC), p38 MAP kinase is a positive regulator of turnover, while PKC is a negative regulator of turnover. Recombinantly expressed murine isozyme CerS1 sensitizes cells to a wide range of drugs including cisplatin, carboplatin, doxorubicin and vincristine, incontrast to isozymes CerS4 and CerS5. The specific effect of CerS1 is mediated through the activation of the MAP kinase p38 Mus musculus
additional information in HEK-293 cells, both endogenous human CerS1 and ectopically expressed murine CerS1 have rapid basal turnover, the turnover requires CerS1 activity and is regulated by the opposing actions of p38 MAP kinase and protein kinase C (PKC), p38 MAP kinase is a positive regulator of turnover, while PKC is a negative regulator of turnover. Recombinant isozyme CerS1 sensitizes cells to a wide range of drugs including cisplatin, carboplatin, doxorubicin and vincristine, in contrast to isozymes CerS4 and CerS5. The specific effect of CerS1 is mediated through the activation of the MAP kinase p38 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
MG132
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Mus musculus
Mg2+ activates Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphinganine + stearoyl-CoA Mus musculus
-
N-stearoylsphinganine + CoA
-
?
sphinganine + stearoyl-CoA Homo sapiens
-
N-stearoylsphinganine + CoA
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens P27544
-
-
Mus musculus P27545
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein CerS1 is phosphorylated in vivo and activation of protein kinase C increases the phosphorylation of the protein Mus musculus
phosphoprotein CerS1 is phosphorylated in vivo and activation of protein kinase C increases the phosphorylation of the protein Homo sapiens
phosphoprotein the enzyme turnover is regulated by the opposing actions of p38 MAP kinase and protein kinase C (PKC). p38 MAP kinase is a positive regulator of turnover, while PKC is a negative regulator of turnover. The enzyme is phosphorylated in vivo and activation of PKC increases the phosphorylation of the protein Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
A-549 cell
-
Homo sapiens
-
HEK-293 cell
-
Homo sapiens
-
HeLa cell
-
Homo sapiens
-
U-373MG cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphinganine + stearoyl-CoA
-
Mus musculus N-stearoylsphinganine + CoA
-
?
sphinganine + stearoyl-CoA
-
Homo sapiens N-stearoylsphinganine + CoA
-
?
stearoyl-CoA + sphinganine
-
Homo sapiens N-(stearoyl)-sphinganine + CoA
-
?

Subunits

Subunits Comment Organism
? x * 39000, FLAG-tagged enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
ceramide synthase 1
-
Homo sapiens
ceramide synthase 1
-
Mus musculus
CerS1
-
Homo sapiens
CerS1
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Mus musculus
7.5
-
assay at Homo sapiens

Expression

Organism Comment Expression
Mus musculus diverse stresses including chemotherapeutic drugs, UV light and DTT can induce CerS1 turnover up
Homo sapiens diverse stresses including chemotherapeutic drugs, UV light and DTT can induce CerS1 turnover up

General Information

General Information Comment Organism
metabolism in HEK-293 cells, both endogenous human CerS1 and ectopically expressed murine CerS1 have rapid basal turnover, the turnover requires CerS1 activity and is regulated by the opposing actions of p38 MAP kinase and protein kinase C (PKC), p38 MAP kinase is a positive regulator of turnover, while PKC is a negative regulator of turnover Mus musculus
metabolism in HEK-293 cells, both endogenous human CerS1 and ectopically expressed murine CerS1 have rapid basal turnover, the turnover requires CerS1 activity and is regulated by the opposing actions of p38 MAP kinase and protein kinase C (PKC), p38 MAP kinase is a positive regulator of turnover, while PKC is a negative regulator of turnover Homo sapiens
physiological function ceramide synthase 1 (CerS1) is the most structurally and functionally distinct from the other CerS enzymes, the enzyme is regulated via a regulatory mechanism that specifically controls the level of CerS1 via ubiquitination and proteasome dependent protein turnover Mus musculus
physiological function ceramide synthase 1 (CerS1) is the most structurally and functionally distinct from the other CerS enzymes, the enzyme is regulated via a regulatory mechanism that specifically controls the level of CerS1 via ubiquitination and proteasome dependent protein turnover Homo sapiens