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Literature summary for 2.3.1.298 extracted from
- 2-Hydroxy-ceramide synthesis by ceramide synthase family enzymatic basis for the preference of FA chain length (2008), J. Lipid Res., 49, 2356-2364 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in HEK-293T cells | Homo sapiens |
| gene CERS3, recombinant expression in HEK-293T cells, quantitative real-time quantitative PCR enzyme expression analysis | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | profiles of non-hydroxylated and 2-hydroxy-ceramides in transgenic HeLa cells expressing different CerS isozymes and or different specific interfence RNAs, overview | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fumonisin B1 | - |
Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| an ultra-long-chain fatty acyl-CoA + a sphingoid base | Homo sapiens | - |
an ultra-long-chain ceramide + CoA | - |
? | |
| sphingosine + behenoyl-CoA | Homo sapiens | - |
N-behenoylsphingosine + CoA | - |
? | |
| sphingosine + eicosanoyl-CoA | Homo sapiens | - |
N-eicosanoylsphingosine + CoA | - |
? | |
| sphingosine + lignoceroyl-CoA | Homo sapiens | - |
N-lignoceroylsphingosine + CoA | - |
? | |
| sphingosine + palmitoyl-CoA | Homo sapiens | - |
N-palmitoylsphingosine + CoA | - |
? | |
| sphingosine + stearoyl-CoA | Homo sapiens | - |
N-stearoylsphingosine + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Homo sapiens | Q8IU89 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epidermis | - |
Homo sapiens | - |
| keratinocyte | - |
Homo sapiens | - |
| keratinocyte | epidermal, expression analysis of the CerS family members during keratinocyte differentiation, overview. Upon differentiation, CerS3 mRNA expression significantly increases and remains high throughout differentiation | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| an ultra-long-chain fatty acyl-CoA + a sphingoid base | - |
Homo sapiens | an ultra-long-chain ceramide + CoA | - |
? | |
| behenoyl-CoA + dihydrosphingosine | - |
Homo sapiens | CoA + N-behenoyldihydrosphingosine | - |
? | |
| lignoceroyl-CoA + dihydrosphingosine | highest activity | Homo sapiens | CoA + N-lignoceroyldihydrosphingosine | - |
? | |
| additional information | isozyme CerS3 exhibits broad substrate specificity toward medium- to long-chain fatty acyl-CoAs (short-chain having less than C18, medium-chain with C18-C22, and long-chain with more than C22). In HEK-293T cells overproducing both CerS3 and 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase (FA2H), no 2-hydroxy-C16:0-ceramide is detected, but both medium- and long-chain 2-hydroxy-ceramides. CerS3 may use both medium- and long-chain 2-hydroxy-fatty acyl-CoAs as substrates | Homo sapiens | ? | - |
? | |
| additional information | the enzyme can also use both medium- and long-chain 2-hydroxy-fatty acyl-CoAs as substrates | Homo sapiens | ? | - |
? | |
| sphingosine + 2-hydroxybehenoyl-CoA | - |
Homo sapiens | N-2-hydroxybehenoylsphingosine + CoA | - |
? | |
| sphingosine + 2-hydroxyeicosanoyl-CoA | - |
Homo sapiens | N-2-hydroxyeicosanoylsphingosine + CoA | - |
? | |
| sphingosine + 2-hydroxylignoceroyl-CoA | - |
Homo sapiens | N-2-hydroxylignoceroylsphingosine + CoA | - |
? | |
| sphingosine + 2-hydroxypalmitoyl-CoA | - |
Homo sapiens | N-2-hydroxypalmitoylsphingosine + CoA | - |
? | |
| sphingosine + 2-hydroxystearoyl-CoA | - |
Homo sapiens | N-2-hydroxystearoylsphingosine + CoA | - |
? | |
| sphingosine + behenoyl-CoA | - |
Homo sapiens | N-behenoylsphingosine + CoA | - |
? | |
| sphingosine + eicosanoyl-CoA | - |
Homo sapiens | N-eicosanoylsphingosine + CoA | - |
? | |
| sphingosine + lignoceroyl-CoA | - |
Homo sapiens | N-lignoceroylsphingosine + CoA | - |
? | |
| sphingosine + palmitoyl-CoA | - |
Homo sapiens | N-palmitoylsphingosine + CoA | - |
? | |
| sphingosine + stearoyl-CoA | - |
Homo sapiens | N-stearoylsphingosine + CoA | - |
? | |
| stearoyl-CoA + dihydrosphingosine | - |
Homo sapiens | CoA + N-stearoyldihydrosphingosine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ceramide synthase 3 | - |
Homo sapiens |
| CerS3 | - |
Homo sapiens |
| LASS3 | - |
Homo sapiens |
| longevity assurance homologue 3 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Homo sapiens | enzyme mRNA expression significantly increases and remains high throughout keratinocyte differentiation | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | profiles of non-hydroxylated and 2-hydroxy-ceramides in transgenic HeLa cells expressing different CerS isozymes and or different specific interfence RNAs, overview | Homo sapiens |
| physiological function | isozyme CerS3 may be important in ceramide and 2-hydroxy-ceramide synthesis in epidermis. Differences in the expression patterns of CerS family members may play an important role in the production of the CER/2-hydroxy ceramide (CER) compositions of different chain lengths observed in different cell types and even in the altered production that occurring during keratinocyte differentiation | Homo sapiens |
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Release 2023.1 (January 2023)
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