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Literature summary for 2.3.1.297 extracted from
- Lip1p a novel subunit of acyl-CoA ceramide synthase (2005), EMBO J., 24, 730-741 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes LAC1, LAG1, and LIP1. LIP1 DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of all three subunits as N-terminally FLAG- or HA-tagged proteins in Saccharomyces cerevisiae strains | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| australifungin | - |
Saccharomyces cerevisiae |  |
| fumonisin B1 | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum membrane | Lac1p and Lag1p are integral membrane proteins with six to eight predicted transmembrane domains. Co-subunit Lip1 is also an integral membrane protein, with one transmembrane domain. Its short N-terminal part is cytoplasmic and not required for ceramide synthesis. Analysis of membrane topology of the heterotrimer for ceramide synthase catalytic activity, overview | Saccharomyces cerevisiae | 5789 | - |
| microsome | - |
Saccharomyces cerevisiae | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-erythro-sphinganine + arachidoyl-CoA | Saccharomyces cerevisiae | - |
N-arachidoyl-D-sphinganine + CoA | - |
? | |
| D-erythro-sphinganine + arachidoyl-CoA | Saccharomyces cerevisiae RH4838 | - |
N-arachidoyl-D-sphinganine + CoA | - |
? | |
| D-erythro-sphinganine + cerotoyl-CoA | Saccharomyces cerevisiae | - |
N-cerotoyl-D-sphinganine + CoA | - |
? | |
| D-erythro-sphinganine + cerotoyl-CoA | Saccharomyces cerevisiae RH4838 | - |
N-cerotoyl-D-sphinganine + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P28496 AND P38703 AND Q03579 | encoding subunits Lac1p, Lag1p, and Lip1p | - |
| Saccharomyces cerevisiae RH4838 | P28496 AND P38703 AND Q03579 | encoding subunits Lac1p, Lag1p, and Lip1p | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 3420fold subunits from isolated digitonin-treated membranes by solubilization with 1% Triton X-100 and affinity chromatography | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20.68 | - |
purified native enzyme, pH 6.8, 24°C | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-erythro-sphinganine + arachidoyl-CoA | - |
Saccharomyces cerevisiae | N-arachidoyl-D-sphinganine + CoA | - |
? | |
| D-erythro-sphinganine + arachidoyl-CoA | - |
Saccharomyces cerevisiae RH4838 | N-arachidoyl-D-sphinganine + CoA | - |
? | |
| D-erythro-sphinganine + cerotoyl-CoA | - |
Saccharomyces cerevisiae | N-cerotoyl-D-sphinganine + CoA | - |
? | |
| D-erythro-sphinganine + cerotoyl-CoA | - |
Saccharomyces cerevisiae RH4838 | N-cerotoyl-D-sphinganine + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotrimer | subunits Lac1p, Lag1p, and Lip1p | Saccharomyces cerevisiae |
| More | Lag1p, Lac1p and Lip1p comprise the ceramide synthase. Lag1p/Lac1p interacting protein, 28 kDa, Lip1p is an essential component of the ceramide synthase, which forms a heteromeric complex with Lac1p and Lag1p. Lip1p is required for ceramide synthesis in vivo and in vitro. It is possible that Lip1p is modified by O-linked glycosylation. Its short, highly charged N-terminus is cytoplasmic and dispensible for ceramide synthase activity | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acyl-CoA ceramide synthase | - |
Saccharomyces cerevisiae |
| ceramide synthase complex | - |
Saccharomyces cerevisiae |
| Lac1p/Lag1p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Saccharomyces cerevisiae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | Lag1p, Lac1p and Lip1p comprise the ceramide synthase. Lag1p and Lac1p are subunits of the acyl-CoA-dependent ceramide synthase. The other essential component of the ceramide synthase, Lip1p, which forms a heteromeric complex with Lac1p and Lag1p. Lip1p is required for ceramide synthesis in vivo and in vitro | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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