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Literature summary for 2.3.1.297 extracted from

  • Mesicek, J.; Lee, H.; Feldman, T.; Jiang, X.; Skobeleva, A.; Berdyshev, E.V.; Haimovitz-Friedman, A.; Fuks, Z.; Kolesnick, R.
    Ceramide synthases 2, 5, and 6 confer distinct roles in radiation-induced apoptosis in HeLa cells (2010), Cell. Signal., 22, 1300-1307 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene CERS2, recombinant FLAG-tagged isozyme overexpression in HeLa cells, quantitative real-time PCR enzyme expression analysis. CerS2, CerS5, and CerS6 form heterocomplexes in transformed HeLa cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information CerS2 knockdown by shRNA Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information nonlinear fitting and Michaelis-Menten kinetic analyses Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial membrane
-
 Homo sapiens 31966
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphinganine + lignoceroyl-CoA Homo sapiens
-
 N-lignoceroylsphinganine + CoA
-
 ?
sphinganine + nervonoyl-CoA Homo sapiens
-
 N-nervonoylsphinganine + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96G23
-
-

Purification (Commentary)

Purification (Comment) Organism
subcellular fractionation of HeLa cells recombinantly expressing the CerS isozyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphinganine + lignoceroyl-CoA
-
 Homo sapiens N-lignoceroylsphinganine + CoA
-
 ?
sphinganine + nervonoyl-CoA
-
 Homo sapiens N-nervonoylsphinganine + CoA
-
 ?

Synonyms

Synonyms Comment Organism
ceramide synthase 2
-
 Homo sapiens
CerS2
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Expression

Organism Comment Expression
Homo sapiens ionizing radiation (IR) induces de novo synthesis of ceramide by specifically activating CerS isoforms 2, 5, and 6 up

General Information

General Information Comment Organism
malfunction overexpression of CerS2 results in partial protection from IR-induced apoptosis. Knockdown studies determines that CerS2 is responsible for all observable IR-induced C24:0 CerS activity. CerS2 and CerS5 overexpression significantly alters apoptosis Homo sapiens
metabolism regulation of CerS isozymes, overview. The interplay among the CerS proteins takes place in a stress stimulus-, cell type-, and subcellular compartment-specific manner. CerS2 and CerS5 overexpression significantly alters apoptosis. Determination of ionizing radiation-induced mitochondrial ceramide elevations via CerS2, 5, and 6, overview. CerS2 and CerS5 overexpressions defines opposing roles in radiation-induced mitochondrial apoptosis Homo sapiens
physiological function selective tissue and subcellular distribution of the six mammalian CerS isoforms, combined with distinct fatty acyl chain length substrate preferences, implicate differential functions of specific ceramide species in cellular signaling. Ionizing radiation (IR) induces de novo synthesis of ceramide to influence HeLa cell apoptosis by specifically activating isozymes CerS isoforms 2, 5, and 6 that generate opposing anti- and pro-apoptotic ceramides in mitochondrial membranes. Isozyme CerS2 is responsible for all observable IR-induced C24:0 CerS activity. IR-induced CerS-mediated ceramide generation, and subsequent apoptosis, occurs in a cell-type specific manner. CerS2 and CerS5 overexpressions defines opposing roles in radiation-induced mitochondrial apoptosis Homo sapiens