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Literature summary for 2.3.1.295 extracted from

  • Chopra, T.; Banerjee, S.; Gupta, S.; Yadav, G.; Anand, S.; Surolia, A.; Roy, R.P.; Mohanty, D.; Gokhale, R.S.
    Novel intermolecular iterative mechanism for biosynthesis of mycoketide catalyzed by a bimodular polyketide synthase (2008), PLoS Biol., 6, e163 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.295

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
C2226A inactive Mycobacterium tuberculosis
S2672A inactive Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
431000
-
 gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a medium-chain acyl-CoA + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+ Mycobacterium tuberculosis
-
 a mycoketide-CoA + 10 CO2 + 10 CoA + 22 NADP+ + 11 H2O
-
 ?
a medium-chain acyl-CoA + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+ Mycobacterium tuberculosis H37Rv
-
 a mycoketide-CoA + 10 CO2 + 10 CoA + 22 NADP+ + 11 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Resource-Q chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
9-hydroxy-decanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?
9-hydroxy-decanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
a medium-chain acyl-CoA + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis a mycoketide-CoA + 10 CO2 + 10 CoA + 22 NADP+ + 11 H2O
-
 ?
a medium-chain acyl-CoA + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis H37Rv a mycoketide-CoA + 10 CO2 + 10 CoA + 22 NADP+ + 11 H2O
-
 ?
decanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?
dodecanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?
dodecanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
hexadecanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?
hexadecanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
hexanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?
hexanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
octadecanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?
octanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?
propionyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?
tetradecanoyl N-acetyl-cysteamine thioester + 5 malonyl-CoA + 5 (S)-methylmalonyl-CoA + 22 NADPH + 22 H+
-
 Mycobacterium tuberculosis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 212000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
pks12
-
 Mycobacterium tuberculosis
polyketide synthase 12
-
 Mycobacterium tuberculosis