Literature summary for 2.3.1.294 extracted from

Sridharan, S.; Wang, L.; Brown, A.; Dover, L.; Kremer, L.; Besra, G.; Sacchettini, J.
X-ray crystal structure of Mycobacterium tuberculosis beta-ketoacyl acyl carrier protein synthase II (mtKasB) (2007), J. Mol. Biol., 366, 469-480 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta (DE3) cells	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with palmitoyl-CoA, sitting drop vapor diffusion method, using 100 mM CAPS pH 10.5, 20 % w/v PEG 8000, 200 mM NaCl, spermine hydrochloride and foscholine-9	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
88000	-	gel filtration	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]	Mycobacterium tuberculosis	-	an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]	-	?
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]	Mycobacterium tuberculosis H37Rv	-	an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-
Mycobacterium tuberculosis H37Rv	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-charged HiTrap chelating agarose column chromatography and Superdex 200 gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]	-	Mycobacterium tuberculosis	an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]	-	?
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]	-	Mycobacterium tuberculosis H37Rv	an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]	-	?
palmitoyl-CoA + a malonyl-[acyl-carrier protein]	-	Mycobacterium tuberculosis	?	-	?
palmitoyl-CoA + a malonyl-[acyl-carrier protein]	-	Mycobacterium tuberculosis H37Rv	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
beta-ketoacyl acyl carrier protein synthase II	-	Mycobacterium tuberculosis
KasB	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)