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Literature summary for 2.3.1.294 extracted from
- The condensing activities of the Mycobacterium tuberculosis type II fatty acid synthase are differentially regulated by phosphorylation (2006), J. Biol. Chem., 281, 30094-30103 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)pLysS and Mycobacterium bovis BCG | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
malonyl-[acyl-carrier protein M] | at pH 7.0 and 37°C | Mycobacterium tuberculosis | |
| 0.0119 | - |
palmitoyl-[acyl-carrier protein M] | at pH 7.0 and 37°C | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] | Mycobacterium tuberculosis | - |
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | - |
? |  |
| an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] | Mycobacterium tuberculosis H37Rv | - |
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis H37Rv | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | phosphorylation increases the activity of the enzyme in the elongation process of long chain fatty acids synthesis | Mycobacterium tuberculosis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione-Sepharose 4B column chromatography and Ni-NTA agarose bead chromatography | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] | - |
Mycobacterium tuberculosis | an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | - |
? | |
| an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] | - |
Mycobacterium tuberculosis H37Rv | an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | - |
? | |
| palmitoyl-[acyl-carrier protein M] + malonyl-[acyl-carrier protein M] | - |
Mycobacterium tuberculosis | ? | - |
? | |
| palmitoyl-[acyl-carrier protein M] + malonyl-[acyl-carrier protein M] | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46400, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-ketoacyl AcpM synthase | - |
Mycobacterium tuberculosis |
| KasB | - |
Mycobacterium tuberculosis |
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Release 2023.1 (January 2023)
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