Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces orinoci | B4ER92 and B4ER95 and B4ER96 and B4ER97 | B4ER92 i.e. subunit NorA, B4ER95 i.e. subunit NorA', B4ER96 i.e. subunit NorB, B4ER97 i.e. subunit NorC | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrobenzoyl-CoA + malonyl-CoA + 6 (S)-methylmalonyl-CoA + 6 NADPH + 4 H+ | - |
Streptomyces orinoci | demethyldeoxyspectinabilin + 7 CO2 + 8 CoA + 6 NADP+ + 5 H2O | - |
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General Information | Comment | Organism |
---|---|---|
physiological function | enzyme generates the backbone of the antibiotic spectinabilin. It is composed of 6 modules and is encoded by four genes. The enzyme extends 4-nitrobenzoyl-CoA by 6 molecules of (S)-methylmalonyl-CoA and a single molecule of malonyl-CoA. The first module (encoded by norA) is used twice in an iterative fashion, so that the seven Claisen condensation reactions are catalysed by only six modules. The iteration becomes possible by the transfer of the [acp]-bound polyketide intermediate back to the ketosynthase (KS) domain on the opposite polyketide synthase strand (polyketides are homodimeric). Comparison of Stretomyces thiolueus aureothin and Streptomyces orinoci neoaureothin gene clusters and enzymes | Streptomyces orinoci |