Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.286 extracted from

  • Parsons, X.H.; Garcia, S.N.; Pillus, L.; Kadonaga, J.T.
    Histone deacetylation by Sir2 generates a transcriptionally repressed nucleoprotein complex (2003), Proc. Natl. Acad. Sci. USA, 100, 1609-1614 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.286

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
coumermycin A1 completely inhibited by 0.2 mM Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hyperacetylated histone-DNA complex + NAD+ + H2O Drosophila melanogaster
-
 deacetylated nuclease-resistant histone-DNA complex + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q9VK34
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity column chromatography and Superdex S200 gel filtration Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hyperacetylated histone-DNA complex + NAD+ + H2O
-
 Drosophila melanogaster deacetylated nuclease-resistant histone-DNA complex + ?
-
 ?
[histone H2B]-N6-acetyl-L-lysine20 + NAD+ + H2O
-
 Drosophila melanogaster [histone H2B]-L-lysine20 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?
[histone H2B]-N6-acetyl-L-lysine5 + NAD+ + H2O
-
 Drosophila melanogaster [histone H2B]-L-lysine5 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?
[histone H3]-N6-acetyl-L-lysine14 + NAD+ + H2O
-
 Drosophila melanogaster [histone H3]-L-lysine14 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?
[histone H3]-N6-acetyl-L-lysine18 + NAD+ + H2O
-
 Drosophila melanogaster [histone H3]-L-lysine18 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?
[histone H3]-N6-acetyl-L-lysine23 + NAD+ + H2O
-
 Drosophila melanogaster [histone H3]-L-lysine23 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?
[histone H3]-N6-acetyl-L-lysine9 + NAD+ + H2O
-
 Drosophila melanogaster [histone H3]-L-lysine9 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?
[histone H4]-N6-acetyl-L-lysine12 + NAD+ + H2O
-
 Drosophila melanogaster [histone H4]-L-lysine12 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?
[histone H4]-N6-acetyl-L-lysine16 + NAD+ + H2O
-
 Drosophila melanogaster [histone H4]-L-lysine16 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?
[histone H4]-N6-acetyl-L-lysine5 + NAD+ + H2O
-
 Drosophila melanogaster [histone H4]-L-lysine5 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?
[histone H4]-N6-acetyl-L-lysine8 + NAD+ + H2O weak activity Drosophila melanogaster [histone H4]-L-lysine8 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?

Subunits

Subunits Comment Organism
? x * 116000, SDS-PAGE Drosophila melanogaster

Synonyms

Synonyms Comment Organism
NAD-dependent histone deacetylase
-
 Drosophila melanogaster
Sir2
-
 Drosophila melanogaster
SIRT1
-
 Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Drosophila melanogaster

General Information

General Information Comment Organism
physiological function the enzyme does not repress transcription with either naked DNA templates or chromatin assembled from native (and mostly unacetylated) histones Drosophila melanogaster