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Literature summary for 2.3.1.286 extracted from

  • Sanders, B.D.; Zhao, K.; Slama, J.T.; Marmorstein, R.
    Structural basis for nicotinamide inhibition and base exchange in Sir2 enzymes (2007), Mol. Cell, 25, 463-472 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in complex with its acetyl-lysine 16 histone H4 substrate, nicotinamide and the reaction intermediate analog ADP-HPD, vapor diffusion method, using 2.0 M (NH4)2SO4, 100 mM Na citrate, pH 5.6, 200 mM K/Na tartrate or 2.0 M (NH4)2SO4 and 100 mM Na citrate, pH 5.5 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D118N the mutant retains approximately 13% of the activity of the wild type protein Saccharomyces cerevisiae
I117A inactive Saccharomyces cerevisiae
I117D inactive Saccharomyces cerevisiae
I117F the mutant is nearly as active as the wild type protein (about 75% activity) Saccharomyces cerevisiae
I117H inactive Saccharomyces cerevisiae
I117L the mutant retains approximately 25% of the activity of the wild type protein Saccharomyces cerevisiae
I117V the mutant is nearly as active as the wild type protein (about 65% activity) Saccharomyces cerevisiae
I117W inactive Saccharomyces cerevisiae
I117Y inactive Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
nicotinamide noncompetitive inhibitor Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0161
-
NAD+ wild type enzyme, at pH 8.0, temperature not specified in the publication Saccharomyces cerevisiae
0.0255
-
NAD+ mutant enzyme I117V, at pH 8.0, temperature not specified in the publication Saccharomyces cerevisiae
0.0258
-
NAD+ mutant enzyme I117F, at pH 8.0, temperature not specified in the publication Saccharomyces cerevisiae
0.4535
-
NAD+ mutant enzyme D118N, at pH 8.0, temperature not specified in the publication Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P53686
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fluorogenic acetyl-L-lysine + NAD+ + H2O
-
Saccharomyces cerevisiae ?
-
?
[histone H4]-N6-acetyl-L-lysine16 + NAD+ + H2O
-
Saccharomyces cerevisiae [histone H4]-L-lysine16 + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
?

Synonyms

Synonyms Comment Organism
HST2
-
Saccharomyces cerevisiae
Sir2
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Saccharomyces cerevisiae