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Literature summary for 2.3.1.286 extracted from

  • Min, J.; Landry, J.; Sternglanz, R.; Xu, R.M.
    Crystal structure of a SIR2 homolog-NAD complex (2001), Cell, 105, 269-279 .
    View publication on PubMed
Search for more literature for 2.3.1.286

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL1Blue cells Archaeoglobus fulgidus

Crystallization (Commentary)

Crystallization (Comment) Organism
isoform SIR2-Af1 in complex with NAD+, hanging drop vapor diffusion method, using 100 mM MES (pH 6.0), and 12% (w/v) PEG-10K Archaeoglobus fulgidus

Protein Variants

Protein Variants Comment Organism
E186A inactive Archaeoglobus fulgidus
H135A inactive Archaeoglobus fulgidus
Q115A the mutant shows very weak deacetylation activity compared to the wild type enzyme Archaeoglobus fulgidus
S36A inactive Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
o-phenanthroline
-
 Archaeoglobus fulgidus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the small domain of the enzyme contains a three-stranded zinc ribbon motif. No exogenous added Zn ions are needed for in vitro deacetylase and nicotinamide-NAD+ exchange activity Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[protein]-N6-acetyl-L-lysine + NAD+ + H2O Archaeoglobus fulgidus
-
 [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Mono S column chromatography, and Superdex S200 gel filtration Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[bovine serum albumin]-N6-acetyl-L-lysine + NAD+ + H2O
-
 Archaeoglobus fulgidus [bovine serum albumin]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?
[protein]-N6-acetyl-L-lysine + NAD+ + H2O
-
 Archaeoglobus fulgidus [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?

Synonyms

Synonyms Comment Organism
NAD-dependent protein deacetylase
-
 Archaeoglobus fulgidus
SIR2-Af1
-
 Archaeoglobus fulgidus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Archaeoglobus fulgidus