Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.286 extracted from

  • French, J.B.; Cen, Y.; Sauve, A.A.
    Plasmodium falciparum Sir2 is an NAD+-dependent deacetylase and an acetyllysine-dependent and acetyllysine-independent NAD+ glycohydrolase (2008), Biochemistry, 47, 10227-10239 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.286

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Codon-plus RIPL cells Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
alpha-1-O-methyl-adenosine diphosphoribose
-
 Plasmodium falciparum
nicotinamide
-
 Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.085
-
 [peptide p300]-N6-acetyl-L-lysine at pH 8.0 and 37°C Plasmodium falciparum
0.176
-
 [histone H4]-N6-acetyl-L-lysine at pH 8.0 and 37°C Plasmodium falciparum
0.372
-
 [histone H3]-N6-acetyl-L-lysine at pH 8.0 and 37°C Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-column affinity chromatography Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[histone H3]-N6-acetyl-L-lysine + NAD+ + H2O histone H3 with the sequence ARTKQTAR(K-Ac)STGG(K-Ac)APRKQLAS. The enzyme deacetylates K9 and K16 of diacetylated histone H3 Plasmodium falciparum [histone H3]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?
[histone H4]-N6-acetyl-L-lysine + NAD+ + H2O histone H3 with the sequence SGRG(K-Ac)GG(K-Ac)GLG(K-Ac)GGA(K-Ac)RHR. The enzyme predominantly deacetylates at K16 of tetra-acetylated histone H4 Plasmodium falciparum [histone H4]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?
[peptide p300]-N6-acetyl-L-lysine + NAD+ + H2O peptide p300 with the sequence ERSTEL(K-Ac)TEI(K-Ac)EEEDQPSTS. The enzyme predominantly deacetylates at K1040 of full length peptide p300 Plasmodium falciparum [histone H4]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?

Synonyms

Synonyms Comment Organism
Sir2
-
 Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00025
-
 [histone H3]-N6-acetyl-L-lysine at pH 8.0 and 37°C Plasmodium falciparum
0.00035
-
 [histone H4]-N6-acetyl-L-lysine at pH 8.0 and 37°C Plasmodium falciparum
0.00092
-
 [peptide p300]-N6-acetyl-L-lysine at pH 8.0 and 37°C Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.035
-
 nicotinamide with [histone H3]-N6-acetyl-L-lysine as substrate, at pH 8.0 and 37°C Plasmodium falciparum
0.091
-
 nicotinamide with [peptide p300]-N6-acetyl-L-lysine as substrate, at pH 8.0 and 37°C Plasmodium falciparum