Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.282 extracted from

  • Onwueme, K.C.; Ferreras, J.A.; Buglino, J.; Lima, C.D.; Quadri, L.E.
    Mycobacterial polyketide-associated proteins are acyltransferases proof of principle with Mycobacterium tuberculosis PapA5 (2004), Proc. Natl. Acad. Sci. USA, 101, 4608-4613 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.282

Protein Variants

Protein Variants Comment Organism
D128A mutation in HHx3DGx13Y motif, loss of activity Mycobacterium tuberculosis
H123A about 1.7- to 2.3fold decrease in ester formation Mycobacterium tuberculosis
H124A mutation in HHx3DGx13Y motif, loss of activity Mycobacterium tuberculosis
Y143F about 1.9fold decrease in ester formation Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
octan-1-ol substrate inhibition Mycobacterium tuberculosis
palmitoyl-CoA substrate inhibition Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 palmitoyl-CoA pH 6.5, 387°C Mycobacterium tuberculosis
0.5
-
 octan-1-ol pH 6.5, 387°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WIN5
-
-
Mycobacterium tuberculosis ATCC 25618 P9WIN5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-octan-2-ol + palmitoyl-CoA
-
 Mycobacterium tuberculosis (R)-2-octanyl palmitate + CoA
-
 ?
(R)-octan-2-ol + palmitoyl-CoA
-
 Mycobacterium tuberculosis ATCC 25618 (R)-2-octanyl palmitate + CoA
-
 ?
(R,S)-octan-2-ol + palmitoyl-CoA
-
 Mycobacterium tuberculosis (R,S)-2-octanyl palmitate + CoA
-
 ?
(R,S)-octan-2-ol + palmitoyl-CoA
-
 Mycobacterium tuberculosis ATCC 25618 (R,S)-2-octanyl palmitate + CoA
-
 ?
decan-1-ol + palmitoyl-CoA
-
 Mycobacterium tuberculosis 1-decyl palmitate + CoA
-
 ?
decan-1-ol + palmitoyl-CoA
-
 Mycobacterium tuberculosis ATCC 25618 1-decyl palmitate + CoA
-
 ?
hexadecanol + palmitoyl-CoA
-
 Mycobacterium tuberculosis hexadecyl palmitate + CoA
-
 ?
hexadecanol + palmitoyl-CoA
-
 Mycobacterium tuberculosis ATCC 25618 hexadecyl palmitate + CoA
-
 ?
additional information enzyme shows a preference for saturated medium chain alcohols, with 1-octanol displaying the highest ester formation stoichiometry. 20- and 5fold more ester is formed with (R)-(-)2-octanol and (S)-(-)1,2-decanediol, respectively, than with their corresponding enantiomers Mycobacterium tuberculosis ?
-
 ?
additional information enzyme shows a preference for saturated medium chain alcohols, with 1-octanol displaying the highest ester formation stoichiometry. 20- and 5fold more ester is formed with (R)-(-)2-octanol and (S)-(-)1,2-decanediol, respectively, than with their corresponding enantiomers Mycobacterium tuberculosis ATCC 25618 ?
-
 ?
nonan-1-ol + palmitoyl-CoA
-
 Mycobacterium tuberculosis 1-nonyl palmitate + CoA
-
 ?
octan-1-ol + elaidoyl-CoA
-
 Mycobacterium tuberculosis 1-octyl elaidoate + CoA
-
 ?
octan-1-ol + heptadecanoyl-CoA
-
 Mycobacterium tuberculosis 1-octyl heptadecanoate + CoA
-
 ?
octan-1-ol + lauroyl-CoA
-
 Mycobacterium tuberculosis 1-octyl laurate + CoA
-
 ?
octan-1-ol + oleoyl-CoA
-
 Mycobacterium tuberculosis 1-octyl oleate + CoA
-
 ?
octan-1-ol + palmitoyl-CoA
-
 Mycobacterium tuberculosis 1-octyl palmitate + CoA
-
 ?
octan-1-ol + stearoyl-CoA
-
 Mycobacterium tuberculosis 1-octyl stearate + CoA
-
 ?

Synonyms

Synonyms Comment Organism
papA5
-
 Mycobacterium tuberculosis
polyketide-associated protein A5
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0004
-
 octan-1-ol pH 6.5, 387°C Mycobacterium tuberculosis
0.00045
-
 palmitoyl-CoA pH 6.5, 387°C Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0096
-
 palmitoyl-CoA pH 6.5, 37°C Mycobacterium tuberculosis
3
-
 octan-1-ol pH 6.5, 37°C Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function PapA5 catalyzes diesterification of phthiocerol and phthiodiolone with mycocerosate. Presence of PapA5 is required for synthesis of phthiocerol and phthiodiolone dimycocerosate esters Mycobacterium tuberculosis