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Literature summary for 2.3.1.282 extracted from

  • Touchette, M.H.; Bommineni, G.R.; Delle Bovi, R.J.; Gadbery, J.E.; Nicora, C.D.; Shukla, A.K.; Kyle, J.E.; Metz, T.O.; Martin, D.W.; Sampson, N.S.; Miller, W.T.; Tonge, P.J.; Seeliger, J.C.
    Diacyltransferase activity and chain length specificity of Mycobacterium tuberculosis PapA5 in the synthesis of alkyl beta-diol lipids (2015), Biochemistry, 54, 5457-5468 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
A382F almost complete loss of activity Mycobacterium tuberculosis
A382M 35% of wild-type activity with substrate 1-octanol, 39% with 5R,7R-undecane-5,7-diol, 48% with pentadecane-5,7-diol, respectively Mycobacterium tuberculosis
G129L 13% of wild-type activity with substrate 1-octanol, 4% with 5R,7R-undecane-5,7-diol, 13% with pentadecane-5,7-diol, respectively Mycobacterium tuberculosis
Q19K 16% of wild-type activity with substrate 1-octanol, 11% with pentadecane-5,7-diol, respectively Mycobacterium tuberculosis
Q19R 68% of wild-type activity with substrate 1-octanol, 48% with 5R,7R-undecane-5,7-diol, 45% with pentadecane-5,7-diol, respectively Mycobacterium tuberculosis
S380M almost complete loss of activity Mycobacterium tuberculosis
V16C/G328C 8% of wild-type activity with with 5R,7R-undecane-5,7-diol, 3% with pentadecane-5,7-diol, respectively Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WIN5
-
-
Mycobacterium tuberculosis H37Rv P9WIN5
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein Ser/Thr kinases PknB and PknE modify PapA5 on three overlapping Thr residues and a fourth Thr is unique to PknE phosphorylation Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
219
-
wild-type, substrate 1-octanol, pH 6.5, 22°C Mycobacterium tuberculosis
311
-
wild-type, substrate 5R,7R-undecane-5,7-diol, pH 6.5, 22°C Mycobacterium tuberculosis
454
-
wild-type, substrate 5R,7R-pentadecane-5,7-diol, pH 6.5, 22°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 palmitoyl-CoA + 5R,7R-pentadecane-5,7-diol
-
Mycobacterium tuberculosis 5,7-dipalmitoyl 5R,7R-pentadecane-5,7-diol + 2 CoA
-
?
2 palmitoyl-CoA + 5R,7R-pentadecane-5,7-diol
-
Mycobacterium tuberculosis H37Rv 5,7-dipalmitoyl 5R,7R-pentadecane-5,7-diol + 2 CoA
-
?
2 palmitoyl-CoA + 5R,7R-undecane-5,7-diol
-
Mycobacterium tuberculosis 5,7-dipalmitoyl 5R,7R-undecane-5,7-diol + 2 CoA
-
?
2 palmitoyl-CoA + 5R,7R-undecane-5,7-diol
-
Mycobacterium tuberculosis H37Rv 5,7-dipalmitoyl 5R,7R-undecane-5,7-diol + 2 CoA
-
?
palmitoyl-CoA + 1-octanol
-
Mycobacterium tuberculosis palmitoyl octanol + 2 CoA
-
?
palmitoyl-CoA + 1-octanol
-
Mycobacterium tuberculosis H37Rv palmitoyl octanol + 2 CoA
-
?

General Information

General Information Comment Organism
physiological function PapA5 is the terminal enzyme in dimycocerosate biosynthesis with dual esterification activity Mycobacterium tuberculosis