Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
A382F | almost complete loss of activity | Mycobacterium tuberculosis |
A382M | 35% of wild-type activity with substrate 1-octanol, 39% with 5R,7R-undecane-5,7-diol, 48% with pentadecane-5,7-diol, respectively | Mycobacterium tuberculosis |
G129L | 13% of wild-type activity with substrate 1-octanol, 4% with 5R,7R-undecane-5,7-diol, 13% with pentadecane-5,7-diol, respectively | Mycobacterium tuberculosis |
Q19K | 16% of wild-type activity with substrate 1-octanol, 11% with pentadecane-5,7-diol, respectively | Mycobacterium tuberculosis |
Q19R | 68% of wild-type activity with substrate 1-octanol, 48% with 5R,7R-undecane-5,7-diol, 45% with pentadecane-5,7-diol, respectively | Mycobacterium tuberculosis |
S380M | almost complete loss of activity | Mycobacterium tuberculosis |
V16C/G328C | 8% of wild-type activity with with 5R,7R-undecane-5,7-diol, 3% with pentadecane-5,7-diol, respectively | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WIN5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WIN5 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | Ser/Thr kinases PknB and PknE modify PapA5 on three overlapping Thr residues and a fourth Thr is unique to PknE phosphorylation | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
219 | - |
wild-type, substrate 1-octanol, pH 6.5, 22°C | Mycobacterium tuberculosis |
311 | - |
wild-type, substrate 5R,7R-undecane-5,7-diol, pH 6.5, 22°C | Mycobacterium tuberculosis |
454 | - |
wild-type, substrate 5R,7R-pentadecane-5,7-diol, pH 6.5, 22°C | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 palmitoyl-CoA + 5R,7R-pentadecane-5,7-diol | - |
Mycobacterium tuberculosis | 5,7-dipalmitoyl 5R,7R-pentadecane-5,7-diol + 2 CoA | - |
? | |
2 palmitoyl-CoA + 5R,7R-pentadecane-5,7-diol | - |
Mycobacterium tuberculosis H37Rv | 5,7-dipalmitoyl 5R,7R-pentadecane-5,7-diol + 2 CoA | - |
? | |
2 palmitoyl-CoA + 5R,7R-undecane-5,7-diol | - |
Mycobacterium tuberculosis | 5,7-dipalmitoyl 5R,7R-undecane-5,7-diol + 2 CoA | - |
? | |
2 palmitoyl-CoA + 5R,7R-undecane-5,7-diol | - |
Mycobacterium tuberculosis H37Rv | 5,7-dipalmitoyl 5R,7R-undecane-5,7-diol + 2 CoA | - |
? | |
palmitoyl-CoA + 1-octanol | - |
Mycobacterium tuberculosis | palmitoyl octanol + 2 CoA | - |
? | |
palmitoyl-CoA + 1-octanol | - |
Mycobacterium tuberculosis H37Rv | palmitoyl octanol + 2 CoA | - |
? |
General Information | Comment | Organism |
---|---|---|
physiological function | PapA5 is the terminal enzyme in dimycocerosate biosynthesis with dual esterification activity | Mycobacterium tuberculosis |