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Literature summary for 2.3.1.272 extracted from

  • Hayashi, A.; Saitou, H.; Mori, T.; Matano, I.; Sugisaki, H.; Maruyama, K.
    Molecular and catalytic properties of monoacetylphloroglucinol acetyltransferase from Pseudomonas sp. YGJ3 (2012), Biosci. Biotechnol. Biochem., 76, 559-566 .
    View publication on PubMed
Search for more literature for 2.3.1.272

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL1-Blue cells Pseudomonas sp. YGJ3

Inhibitors

Inhibitors Comment Organism Structure
2,4-diacetylphloroglucinol 2,4-diacetylphloroglucinol inhibits the forward reaction competitively Pseudomonas sp. YGJ3
Cl- inhibited by 2 mM Cl- Pseudomonas sp. YGJ3
diethylpyrocarbonate 88% inhibition at 1 mM Pseudomonas sp. YGJ3
Hg2+ complete inhibition at 0.1 mM Pseudomonas sp. YGJ3
additional information NaCl, MgCl2, CaCl2,FeCl3, ZnCl2, CuCl2, and NiCl2, and various metal chelators (0.5 mM), including 1,10-phenanthroline, 8-hydroxyquinoline, 2,2'-dipyridine, and catechol-3,5-disulfonic acid disodium salt, have almost no effect on enzyme activity. Neither activation nor inhibition is found with thiols (1mM) such as L-cysteine, 2-mercaptoethanol, and dithiothreitol; not inhibited by 2 mM Br- or 2 mM I-, as well as phloroglucinol, acetyl-CoA, and malonyl-CoA Pseudomonas sp. YGJ3
p-chloromercuribenzoate complete inhibition at 1 mM Pseudomonas sp. YGJ3
pyoluteorin inhibited by 0.05 mM pyoluteorin Pseudomonas sp. YGJ3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 2,4-diacetylphloroglucinol at pH 7.0 and 25°C Pseudomonas sp. YGJ3
0.26
-
 2-acetylphloroglucinol at pH 7.0 and 25°C Pseudomonas sp. YGJ3
0.77
-
 resorcinol at pH 7.0 and 25°C Pseudomonas sp. YGJ3
1.31
-
 phloroglucinol at pH 7.0 and 25°C Pseudomonas sp. YGJ3

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
330000
-
 gel filtration Pseudomonas sp. YGJ3

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 2-acetylphloroglucinol Pseudomonas sp. YGJ3
-
 2,4-diacetylphloroglucinol + phloroglucinol
-
 r
2,4-diacetylphloroglucinol + phloroglucinol Pseudomonas sp. YGJ3
-
 2 2-acetylphloroglucinol
-
 r

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. YGJ3 F7J5Y3 subunit PhlB
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Q Sepharose column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration Pseudomonas sp. YGJ3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 2-acetylphloroglucinol
-
 Pseudomonas sp. YGJ3 2,4-diacetylphloroglucinol + phloroglucinol
-
 r
2,4-diacetylphloroglucinol + phloroglucinol
-
 Pseudomonas sp. YGJ3 2 2-acetylphloroglucinol
-
 r
2,4-diacetylphloroglucinol + resorcinol
-
 Pseudomonas sp. YGJ3 ?
-
 r
additional information no activity with pyrocatechol Pseudomonas sp. YGJ3 ?
-
 ?

Subunits

Subunits Comment Organism
heterotrimer x * 42500 + x * 38500 + x * 16600, calculated from amino acid sequence Pseudomonas sp. YGJ3
heterotrimer x * 43000 + x * 39000 + x * 17000, SDS-PAGE Pseudomonas sp. YGJ3

Synonyms

Synonyms Comment Organism
MAPG acetyltransferase
-
 Pseudomonas sp. YGJ3
MAPG Atase
-
 Pseudomonas sp. YGJ3
monoacetylphloroglucinol acetyltransferase
-
 Pseudomonas sp. YGJ3
PhlA subunit Pseudomonas sp. YGJ3
phlACB
-
 Pseudomonas sp. YGJ3
phlB subunit Pseudomonas sp. YGJ3
PhlC subunit Pseudomonas sp. YGJ3

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 65 the enzyme retains its activity below 40°C for 20 min, but is inactivated at above 65°C Pseudomonas sp. YGJ3

pH Range

pH Minimum pH Maximum Comment Organism
6.3 7.2 the forward and the reverse reactions show optimum pH values at about 6.3-7.2 Pseudomonas sp. YGJ3

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.018
-
 2,4-diacetylphloroglucinol at pH 7.0 and 25°C Pseudomonas sp. YGJ3

Expression

Organism Comment Expression
Pseudomonas sp. YGJ3 2 mM Cl- and 0.05 mM pyoluteorin repress the expression of the enzyme down