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Literature summary for 2.3.1.271 extracted from

  • Nasuno, R.; Hirano, Y.; Itoh, T.; Hakoshima, T.; Hibi, T.; Takagi, H.
    Structural and functional analysis of the yeast n-Acetyltransferase mpr1 involved in oxidative stress tolerance via proline metabolism (2013), Proc. Natl. Acad. Sci. USA, 110, 11821-11826 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.271

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) or B834(DE3) cells Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with the substrate cis-4-hydroxy-L-proline, sitting drop vapor diffusion method, using 100 mM Bistris-HCl (pH 5.5), 240 mM MgCl2, and 20.5% (w/v) PEG 3350 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
N135A the mutation causes a remarkable increase in the apparent Km value for acetyl-CoA Saccharomyces cerevisiae
N135D inactive Saccharomyces cerevisiae
N178A the mutation causes a large reduction in the apparent kcat value for acetyl-CoA Saccharomyces cerevisiae
N178D inactive Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
L-azetidine-2-carboxylic acid
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0043
-
 acetyl-CoA wild type enzyme, at pH 7.5 and 30°C Saccharomyces cerevisiae
0.0073
-
 acetyl-CoA mutant enzyme N178A, at pH 7.5 and 30°C Saccharomyces cerevisiae
0.022
-
 acetyl-CoA mutant enzyme N135A, at pH 7.5 and 30°C Saccharomyces cerevisiae
20.9
-
 L-azetidine-2-carboxylic acid wild type enzyme, at pH 7.5 and 30°C Saccharomyces cerevisiae
135.8
-
 L-azetidine-2-carboxylic acid mutant enzyme N178A, at pH 7.5 and 30°C Saccharomyces cerevisiae
407
-
 L-azetidine-2-carboxylic acid mutant enzyme N135A, at pH 7.5 and 30°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae Sigma1278b
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose 6 column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + cis-4-hydroxy-L-proline
-
 Saccharomyces cerevisiae CoA + N-acetyl-cis-4-hydroxy-L-proline
-
 ?
acetyl-CoA + cis-4-hydroxy-L-proline
-
 Saccharomyces cerevisiae Sigma1278b CoA + N-acetyl-cis-4-hydroxy-L-proline
-
 ?
acetyl-CoA + L-azetidine-2-carboxylic acid
-
 Saccharomyces cerevisiae CoA + N-acetyl-L-azetidine-2-carboxylic acid
-
 ?
acetyl-CoA + L-azetidine-2-carboxylic acid
-
 Saccharomyces cerevisiae Sigma1278b CoA + N-acetyl-L-azetidine-2-carboxylic acid
-
 ?
acetyl-CoA + L-DELTA1-pyrroline-5-carboxylate + H2O
-
 Saccharomyces cerevisiae CoA + N-acetyl-glutamate-gamma-semialdehyde
-
 ?
acetyl-CoA + L-DELTA1-pyrroline-5-carboxylate + H2O
-
 Saccharomyces cerevisiae Sigma1278b CoA + N-acetyl-glutamate-gamma-semialdehyde
-
 ?

Synonyms

Synonyms Comment Organism
MPR1
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.2
-
 L-azetidine-2-carboxylic acid mutant enzyme N178A, at pH 7.5 and 30°C Saccharomyces cerevisiae
38.6
-
 L-azetidine-2-carboxylic acid mutant enzyme N135A, at pH 7.5 and 30°C Saccharomyces cerevisiae
287.2
-
 L-azetidine-2-carboxylic acid wild type enzyme, at pH 7.5 and 30°C Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.3
-
 L-azetidine-2-carboxylic acid wild type enzyme, at pH 7.5 and 30°C Saccharomyces cerevisiae