Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
L-azetidine-2-carboxylic acid | at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
7.01 | - |
DELTA1-Pyrroline-5-carboxylate | at pH 7.5 and 30°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | E9P8D2 | - |
- |
Saccharomyces cerevisiae Sigma1278b | E9P8D2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + DELTA1-pyrroline-5-carboxylate + H2O | - |
Saccharomyces cerevisiae | CoA + N-acetyl-glutamate-gamma-semialdehyde | - |
? | |
acetyl-CoA + DELTA1-pyrroline-5-carboxylate + H2O | - |
Saccharomyces cerevisiae Sigma1278b | CoA + N-acetyl-glutamate-gamma-semialdehyde | - |
? | |
acetyl-CoA + L-azetidine-2-carboxylic acid | - |
Saccharomyces cerevisiae | CoA + N-acetyl-L-azetidine-2-carboxylic acid | - |
? | |
acetyl-CoA + L-azetidine-2-carboxylic acid | - |
Saccharomyces cerevisiae Sigma1278b | CoA + N-acetyl-L-azetidine-2-carboxylic acid | - |
? | |
acetyl-CoA + L-glutamate-5-semialdehyde | - |
Saccharomyces cerevisiae | CoA + N-acetyl-L-glutamate 5-semialdehyde | - |
? | |
acetyl-CoA + L-glutamate-5-semialdehyde | - |
Saccharomyces cerevisiae Sigma1278b | CoA + N-acetyl-L-glutamate 5-semialdehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MPR1 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.9 | - |
L-azetidine-2-carboxylic acid | at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
121 | - |
DELTA1-Pyrroline-5-carboxylate | at pH 7.5 and 30°C | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme protects yeast cells from DELTA1-pyrroline-5-carboxylate-induced oxidative stress by reducing reactive oxygen species | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.2 | - |
DELTA1-Pyrroline-5-carboxylate | at pH 7.5 and 30°C | Saccharomyces cerevisiae | |
19.8 | - |
L-azetidine-2-carboxylic acid | at pH 8.5 and 30°C | Saccharomyces cerevisiae |