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Literature summary for 2.3.1.271 extracted from

  • Kotani, T.; Takagi, H.
    Identification of amino acid residues essential for the yeast N-acetyltransferase Mpr1 activity by site-directed mutagenesis (2008), FEMS Yeast Res., 8, 607-614 .
    View publication on PubMed
Search for more literature for 2.3.1.271

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain CKY263 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
R145A the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and acetyl-CoA Saccharomyces cerevisiae
R145D the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and acetyl-CoA Saccharomyces cerevisiae
R145E the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and acetyl-CoA Saccharomyces cerevisiae
R145G the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and acetyl-CoA Saccharomyces cerevisiae
R145W the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and acetyl-CoA Saccharomyces cerevisiae
Y121A the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and increases affinity for acetyl-CoA Saccharomyces cerevisiae
Y157A the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and increases affinity for acetyl-CoA Saccharomyces cerevisiae
Y166A inactive Saccharomyces cerevisiae
Y166F the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and increases affinity for acetyl-CoA Saccharomyces cerevisiae
Y166F the mutation leads to a remarkable decrease of the kcat/Km value for l-azetidine-2-carboxylic acid compared to the wild type enzyme Saccharomyces cerevisiae
Y168A the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and increases affinity for acetyl-CoA Saccharomyces cerevisiae
Y75A the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and acetyl-CoA Saccharomyces cerevisiae
Y89A the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and increases affinity for acetyl-CoA Saccharomyces cerevisiae
Y989A the mutation results in a 2.6fold reduction in the kcat/Km ratio for l-azetidine-2-carboxylic acid compared to the wild type enzyme Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00689
-
 acetyl-CoA mutant enzyme Y168A, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.00761
-
 acetyl-CoA mutant enzyme Y157A, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.0087
-
 acetyl-CoA mutant enzyme Y121A, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.00928
-
 acetyl-CoA mutant enzyme Y166F, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.0179
-
 acetyl-CoA mutant enzyme Y89A, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.0282
-
 acetyl-CoA wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.0456
-
 acetyl-CoA mutant enzyme Y75A, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.569
-
 acetyl-CoA mutant enzyme R145A, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.747
-
 acetyl-CoA mutant enzyme R145W, at pH 8.5 and 30°C Saccharomyces cerevisiae
1.076
-
 acetyl-CoA mutant enzyme R145G, at pH 8.5 and 30°C Saccharomyces cerevisiae
1.17
-
 L-azetidine-2-carboxylic acid mutant enzyme Y168A, at pH 8.5 and 30°C Saccharomyces cerevisiae
1.5 2 L-azetidine-2-carboxylic acid wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.91
-
 L-azetidine-2-carboxylic acid mutant enzyme Y89A, at pH 8.5 and 30°C Saccharomyces cerevisiae
3.144
-
 acetyl-CoA mutant enzyme R145E, at pH 8.5 and 30°C Saccharomyces cerevisiae
3.387
-
 acetyl-CoA mutant enzyme R145D, at pH 8.5 and 30°C Saccharomyces cerevisiae
3.49
-
 L-azetidine-2-carboxylic acid mutant enzyme Y157A, at pH 8.5 and 30°C Saccharomyces cerevisiae
4.03
-
 L-azetidine-2-carboxylic acid mutant enzyme Y166F, at pH 8.5 and 30°C Saccharomyces cerevisiae
12.7
-
 L-azetidine-2-carboxylic acid mutant enzyme Y121A, at pH 8.5 and 30°C Saccharomyces cerevisiae
60.9
-
 L-azetidine-2-carboxylic acid mutant enzyme R145A, at pH 8.5 and 30°C Saccharomyces cerevisiae
64.4
-
 L-azetidine-2-carboxylic acid mutant enzyme R145G, at pH 8.5 and 30°C Saccharomyces cerevisiae
78.4
-
 L-azetidine-2-carboxylic acid mutant enzyme R145W, at pH 8.5 and 30°C Saccharomyces cerevisiae
92.7
-
 L-azetidine-2-carboxylic acid mutant enzyme Y75A, at pH 8.5 and 30°C Saccharomyces cerevisiae
116
-
 L-azetidine-2-carboxylic acid mutant enzyme R145E, at pH 8.5 and 30°C Saccharomyces cerevisiae
192
-
 L-azetidine-2-carboxylic acid mutant enzyme R145D, at pH 8.5 and 30°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae E9P8D2
-
-
Saccharomyces cerevisiae Sigma1278b E9P8D2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-azetidine-2-carboxylic acid
-
 Saccharomyces cerevisiae CoA + N-acetyl-L-azetidine-2-carboxylic acid
-
 ?
acetyl-CoA + L-azetidine-2-carboxylic acid
-
 Saccharomyces cerevisiae Sigma1278b CoA + N-acetyl-L-azetidine-2-carboxylic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 27900, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
MPR1
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the enzyme retains 90% activity after 5 min at 50°C Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.31
-
 L-azetidine-2-carboxylic acid mutant enzyme Y157A, at pH 8.5 and 30°C Saccharomyces cerevisiae
7.09
-
 L-azetidine-2-carboxylic acid mutant enzyme R145D, at pH 8.5 and 30°C Saccharomyces cerevisiae
12.8
-
 L-azetidine-2-carboxylic acid mutant enzyme Y121A, at pH 8.5 and 30°C Saccharomyces cerevisiae
13.7
-
 L-azetidine-2-carboxylic acid mutant enzyme Y166F, at pH 8.5 and 30°C Saccharomyces cerevisiae
19.3
-
 L-azetidine-2-carboxylic acid mutant enzyme Y168A, at pH 8.5 and 30°C Saccharomyces cerevisiae
24.8
-
 L-azetidine-2-carboxylic acid mutant enzyme Y89A, at pH 8.5 and 30°C Saccharomyces cerevisiae
33.5
-
 L-azetidine-2-carboxylic acid wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
63.1
-
 L-azetidine-2-carboxylic acid mutant enzyme R145E, at pH 8.5 and 30°C Saccharomyces cerevisiae
73.6
-
 L-azetidine-2-carboxylic acid mutant enzyme Y75A, at pH 8.5 and 30°C Saccharomyces cerevisiae
225
-
 L-azetidine-2-carboxylic acid mutant enzyme R145W, at pH 8.5 and 30°C Saccharomyces cerevisiae
304
-
 L-azetidine-2-carboxylic acid mutant enzyme R145G, at pH 8.5 and 30°C Saccharomyces cerevisiae
609
-
 L-azetidine-2-carboxylic acid mutant enzyme R145A, at pH 8.5 and 30°C Saccharomyces cerevisiae

General Information

General Information Comment Organism
metabolism the enzyme confers resistance to the proline analogue azetidine-2-carboxylate Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0369
-
 L-azetidine-2-carboxylic acid mutant enzyme R145D, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.548
-
 L-azetidine-2-carboxylic acid mutant enzyme R145E, at pH 8.5 and 30°C Saccharomyces cerevisiae
0.794
-
 L-azetidine-2-carboxylic acid mutant enzyme Y75A, at pH 8.5 and 30°C Saccharomyces cerevisiae
1
-
 L-azetidine-2-carboxylic acid mutant enzyme Y121A, at pH 8.5 and 30°C Saccharomyces cerevisiae
1.24
-
 L-azetidine-2-carboxylic acid mutant enzyme Y157A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.12
-
 acetyl-CoA mutant enzyme R145D, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.88
-
 L-azetidine-2-carboxylic acid mutant enzyme R145W, at pH 8.5 and 30°C Saccharomyces cerevisiae
3.41
-
 L-azetidine-2-carboxylic acid mutant enzyme Y166F, at pH 8.5 and 30°C Saccharomyces cerevisiae
4.72
-
 L-azetidine-2-carboxylic acid mutant enzyme R145G, at pH 8.5 and 30°C Saccharomyces cerevisiae
8.51
-
 L-azetidine-2-carboxylic acid mutant enzyme Y89A, at pH 8.5 and 30°C Saccharomyces cerevisiae
10
-
 L-azetidine-2-carboxylic acid mutant enzyme R145A, at pH 8.5 and 30°C Saccharomyces cerevisiae
16.5
-
 L-azetidine-2-carboxylic acid mutant enzyme Y168A, at pH 8.5 and 30°C Saccharomyces cerevisiae
20.1
-
 acetyl-CoA mutant enzyme R145E, at pH 8.5 and 30°C Saccharomyces cerevisiae
22.4
-
 L-azetidine-2-carboxylic acid wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
283
-
 acetyl-CoA mutant enzyme R145G, at pH 8.5 and 30°C Saccharomyces cerevisiae
302
-
 acetyl-CoA mutant enzyme R145W, at pH 8.5 and 30°C Saccharomyces cerevisiae
569
-
 acetyl-CoA mutant enzyme Y157A, at pH 8.5 and 30°C Saccharomyces cerevisiae
1071
-
 acetyl-CoA mutant enzyme R145A, at pH 8.5 and 30°C Saccharomyces cerevisiae
1190
-
 acetyl-CoA wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
1392
-
 acetyl-CoA mutant enzyme Y89A, at pH 8.5 and 30°C Saccharomyces cerevisiae
1470
-
 acetyl-CoA mutant enzyme Y121A, at pH 8.5 and 30°C Saccharomyces cerevisiae
1481
-
 acetyl-CoA mutant enzyme Y166F, at pH 8.5 and 30°C Saccharomyces cerevisiae
1615
-
 acetyl-CoA mutant enzyme Y75A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2814
-
 acetyl-CoA mutant enzyme Y168A, at pH 8.5 and 30°C Saccharomyces cerevisiae