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Literature summary for 2.3.1.268 extracted from

  • Kruis, A.J.; Levisson, M.; Mars, A.E.; van der Ploeg, M.; Garces Daza, F.; Ellena, V.; Kengen, S.W.M.; van der Oost, J.; Weusthuis, R.A.
    Ethyl acetate production by the elusive alcohol acetyltransferase from yeast (2017), Metab. Eng., 41, 92-101 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae INVSc1 and Escherichia coli BL21 (DE3) cells Wickerhamomyces anomalus

Protein Variants

Protein Variants Comment Organism
D145A mutation in Ser-Asp-His catalytic triad, loss of activity Wickerhamomyces anomalus
H295A mutation in Ser-Asp-His catalytic triad, loss of activity Wickerhamomyces anomalus
S121A mutation in Ser-Asp-His catalytic triad, loss of activity Wickerhamomyces anomalus

Inhibitors

Inhibitors Comment Organism Structure
ethynol inhibitory above 80 mM Wickerhamomyces anomalus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.43
-
acetyl-CoA at pH 7.5 and 30°C Wickerhamomyces anomalus
3.12
-
acetyl-CoA pH 7.5, 30°C Wickerhamomyces anomalus
3.12
-
ethanol at pH 7.5 and 30°C Wickerhamomyces anomalus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethanol + acetyl-CoA Wickerhamomyces anomalus
-
ethyl acetate + CoA
-
?
ethanol + acetyl-CoA Wickerhamomyces anomalus DSM 6766
-
ethyl acetate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Wickerhamomyces anomalus
-
-
-
Wickerhamomyces anomalus A0A1E3P8S6
-
-
Wickerhamomyces anomalus ATCC 58044 A0A1E3P8S6
-
-
Wickerhamomyces anomalus DSM 6766
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and HiTrap SP column chromatography Wickerhamomyces anomalus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + ethanol
-
Wickerhamomyces anomalus CoA + ethyl acetate
-
r
acetyl-CoA + ethanol
-
Wickerhamomyces anomalus ATCC 58044 CoA + ethyl acetate
-
r
ethanol + acetyl-CoA
-
Wickerhamomyces anomalus ethyl acetate + CoA
-
?
ethanol + acetyl-CoA
-
Wickerhamomyces anomalus DSM 6766 ethyl acetate + CoA
-
?
additional information Eat1 can also function as a thioesterase hydrolyzing acetyl-CoA and as esterase hydrolizing ethyl acetate Wickerhamomyces anomalus ?
-
-
additional information Eat1 can also function as a thioesterase hydrolyzing acetyl-CoA and as esterase hydrolizing ethyl acetate Wickerhamomyces anomalus ATCC 58044 ?
-
-

Synonyms

Synonyms Comment Organism
eat1
-
Wickerhamomyces anomalus
ethanol acetyltransferase
-
Wickerhamomyces anomalus
ethanol acetyltransferase 1
-
Wickerhamomyces anomalus
Wanomala_5543
-
Wickerhamomyces anomalus

General Information

General Information Comment Organism
physiological function expression in Saccharomyces cerevisiae and Escherichia coli results in high ethyl acetate production Wickerhamomyces anomalus