Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae INVSc1 and Escherichia coli BL21 (DE3) cells | Wickerhamomyces anomalus |
Protein Variants | Comment | Organism |
---|---|---|
D145A | mutation in Ser-Asp-His catalytic triad, loss of activity | Wickerhamomyces anomalus |
H295A | mutation in Ser-Asp-His catalytic triad, loss of activity | Wickerhamomyces anomalus |
S121A | mutation in Ser-Asp-His catalytic triad, loss of activity | Wickerhamomyces anomalus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethynol | inhibitory above 80 mM | Wickerhamomyces anomalus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.43 | - |
acetyl-CoA | at pH 7.5 and 30°C | Wickerhamomyces anomalus | |
3.12 | - |
acetyl-CoA | pH 7.5, 30°C | Wickerhamomyces anomalus | |
3.12 | - |
ethanol | at pH 7.5 and 30°C | Wickerhamomyces anomalus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + acetyl-CoA | Wickerhamomyces anomalus | - |
ethyl acetate + CoA | - |
? | |
ethanol + acetyl-CoA | Wickerhamomyces anomalus DSM 6766 | - |
ethyl acetate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Wickerhamomyces anomalus | - |
- |
- |
Wickerhamomyces anomalus | A0A1E3P8S6 | - |
- |
Wickerhamomyces anomalus ATCC 58044 | A0A1E3P8S6 | - |
- |
Wickerhamomyces anomalus DSM 6766 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and HiTrap SP column chromatography | Wickerhamomyces anomalus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + ethanol | - |
Wickerhamomyces anomalus | CoA + ethyl acetate | - |
r | |
acetyl-CoA + ethanol | - |
Wickerhamomyces anomalus ATCC 58044 | CoA + ethyl acetate | - |
r | |
ethanol + acetyl-CoA | - |
Wickerhamomyces anomalus | ethyl acetate + CoA | - |
? | |
ethanol + acetyl-CoA | - |
Wickerhamomyces anomalus DSM 6766 | ethyl acetate + CoA | - |
? | |
additional information | Eat1 can also function as a thioesterase hydrolyzing acetyl-CoA and as esterase hydrolizing ethyl acetate | Wickerhamomyces anomalus | ? | - |
- |
|
additional information | Eat1 can also function as a thioesterase hydrolyzing acetyl-CoA and as esterase hydrolizing ethyl acetate | Wickerhamomyces anomalus ATCC 58044 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
eat1 | - |
Wickerhamomyces anomalus |
ethanol acetyltransferase | - |
Wickerhamomyces anomalus |
ethanol acetyltransferase 1 | - |
Wickerhamomyces anomalus |
Wanomala_5543 | - |
Wickerhamomyces anomalus |
General Information | Comment | Organism |
---|---|---|
physiological function | expression in Saccharomyces cerevisiae and Escherichia coli results in high ethyl acetate production | Wickerhamomyces anomalus |