Literature summary for 2.3.1.262 extracted from

Bera, A.K.; Atanasova, V.; Robinson, H.; Eisenstein, E.; Coleman, J.P.; Pesci, E.C.; Parsons, J.F.
Structure of PqsD, a Pseudomonas quinolone signal biosynthetic enzyme, in complex with anthranilate (2009), Biochemistry, 48, 8644-8655.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structures of the enzyme including the PqsD-anthranilate covalent intermediate and the inactive Cys112Ala active site mutant in complex with anthranilate. The crystallographic asymmetric unit contains a PqsD dimer. The PqsD monomer is composed of two nearly identical about 170 residue alphabetalaphabeta domains. Anthranilate-liganded residue Cys112 is positioned deep in the protein interior at the bottom of a 15 A long channel while a second anthraniloyl-CoA molecule is waiting in the cleft leading to the protein surface. Cys112, His257, and Asn287 form the FabH-like catalytic triad of PqsD	Pseudomonas aeruginosa

Crystallization (Comment)

Organism

crystal structures of the enzyme including the PqsD-anthranilate covalent intermediate and the inactive Cys112Ala active site mutant in complex with anthranilate. The crystallographic asymmetric unit contains a PqsD dimer. The PqsD monomer is composed of two nearly identical about 170 residue alphabetalaphabeta domains. Anthranilate-liganded residue Cys112 is positioned deep in the protein interior at the bottom of a 15 A long channel while a second anthraniloyl-CoA molecule is waiting in the cleft leading to the protein surface. Cys112, His257, and Asn287 form the FabH-like catalytic triad of PqsD

Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
C112A	mutant is inactive although it still reversibly binds anthraniloyl-CoA	Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
72800	-	gel filtration	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	P20582	cf. EC 2.3.1.230	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
anthraniloyl-CoA + malonyl-CoA	-	Pseudomonas aeruginosa	2,4-dihydroxyquinoline + 2 CoA + CO2 + ?	in vitro overall reaction	?

Subunits

Subunits	Comment	Organism
dimer	2 * 36379, calculated	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
PqsD	-	Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.3	-	anthraniloyl-CoA	pH 7.5, 37°C	Pseudomonas aeruginosa